Characterization of miRNAs and their target genes in He-Ne laser pretreated wheat seedlings exposed to drought stress.
Characterization of miRNAs and their target genes in He-Ne laser pretreated wheat seedlings exposed to drought stress.
复制标题
氦氖激光预处理的干旱胁迫下小麦幼苗中微小核糖核酸（miRNAs）及其靶基因的特性分析
DOI：
10.1016/j.ecoenv.2018.08.077
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发表时间：
2018-11
影响因子：
6.8
通讯作者：
Z. Qiu;Yanyan He;Yimeng Zhang;Junli Guo;Li Wang
中科院分区：
文献类型：
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作者：
Z. Qiu;Yanyan He;Yimeng Zhang;Junli Guo;Li Wang
干旱胁迫被认为是对小麦生长和发育产生负面影响的关键环境因素，导致全球小麦产量遭受重大损失。最近，人们发现许多 microRNA (miRNA) 参与小麦对干旱胁迫的反应。然而，关于He-Ne激光照射对干旱胁迫下小麦幼苗miRNA及其靶标表达特性的影响的信息很少。因此，在当前的研究中，采用生理学和分子学方法相结合的方法来评估He-Ne激光照射对暴露于干旱胁迫的小麦幼苗中miRNA及其靶标表达的影响。我们的结果表明，干旱胁迫显着降低了小麦幼苗的株高、根长、芽和根鲜重、相对含水量、超氧化物歧化酶（SOD）的表达水平和活性，并增加了丙二醛（MDA）浓度。而氦氖激光照射通过调节SOD、POD、APX基因表达，显着增强了幼苗SOD、抗坏血酸过氧化物酶(APX)、过氧化物酶(POD)的活性和相对含水量，并降低了幼苗的MDA浓度。此外，与单独干旱胁迫相比，He-Ne激光照射的幼苗在干旱胁迫下，miR160、miR164和miR398转录本下调，其靶标生长素反应因子（ARF22）、NAC结构域转录因子和铜/锌超氧化物歧化酶（CSD）表达水平上调。这些结果表明，He-Ne 激光照射可能通过增加 SOD、POD 和 APX 的转录水平和活性，并降低 miR160、miR164 和 miR398 的转录水平，至少部分地保护干旱胁迫。据我们所知，这是第一项提供生化和分子证据支持氦氖激光照射通过 miRNA 表达介导缓解小麦幼苗干旱胁迫效果的研究。
Drought stress is considered a critical environmental factor that negatively affects wheat growth and development, which causes considerable losses in wheat yields worldwide. More recently, numerous microRNAs (miRNAs) have been found to be involved in wheat responses to drought stresses. However, there is little information regarding the effects of He-Ne laser irradiation on the expression traits of miRNAs and their targets in wheat seedlings exposed to drought stress. In the current study, therefore, a combination of physiological and molecular approaches was used to assess the effect of He-Ne laser irradiation on the expression of miRNAs and their targets in wheat seedlings exposed to drought stress. Our results demonstrated that drought stress significantly reduced plant height, root length, shoot and root fresh weight, relative water content, the expression level and activity of superoxide dismutase (SOD), enhanced malondialdehyde (MDA) concentration in the wheat seedlings. However, He-Ne laser irradiation significantly enhanced the activities of SOD, ascorbate peroxidase (APX), peroxidase (POD) and relative water content, and reduced MDA concentration of seedlings by regulating gene expression forSOD,POD,APX. In addition, in comparison with drought stress alone, miR160, miR164 and miR398 transcripts were down-regulated, and expression levels of its targets auxin response factor (ARF22), NAC domain transcription factor and Cu/Zn superoxide dismutases (CSD) were up-regulated in He-Ne laser irradiated seedlings exposed to drought stress. These results suggested that He-Ne laser irradiation could possible protection of drought stress, at least partially, by increasing the transcript levels and activities of SOD, POD and APX, and decreasing the transcript levels of miR160, miR164 and miR398. To the best of our knowledge, this is the first study to present biochemical and molecular evidence supporting the effect of He-Ne laser irradiation on the alleviation of drought stress in wheat seedlings mediated by miRNA expression.