Ectopic expression of the LoERF017 transcription factor from Larix olgensis Henry enhances salt and osmotic-stress tolerance in Arabidopsis thaliana
Ectopic expression of the LoERF017 transcription factor from Larix olgensis Henry enhances salt and osmotic-stress tolerance in Arabidopsis thaliana
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DOI：
10.1007/s11816-018-0473-z
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发表时间：
2018-04-01
影响因子：
2.4
通讯作者：
Li, Chenghao
中科院分区：
文献类型：
--
作者：
Hu, Xiaoqing;Xu, Xuemei;Li, Chenghao
脱水响应元件结合（DREB）转录因子在植物对多种非生物胁迫的响应中起着重要作用，并可调控下游一些防御基因的表达。本研究从长白落叶松中克隆了一个新的DREB TF基因，命名为LoERF 017。LoERF 017基因的开放阅读框为624 bp，含有一个AP 2/EREBP结构域，属于典型的DREB TF。亚细胞定位分析显示LoERF 017定位于细胞核。QRT-PCR分析表明，LoERF 017可以被NaCl、PEG 6000、硝普钠（SNP）和阿坝诱导表达。对转基因植株在甘露醇和NaCl胁迫下的存活率和生长发育的分析表明，LoERF 017基因在拟南芥中的过表达能够提高拟南芥对渗透胁迫和盐胁迫的耐受性。与野生型（WT）相比，LoERF 017的过表达导致了渗透胁迫下超氧化物歧化酶（SOD）和过氧化物酶（POD）活性的提高以及丙二醛（MDA）和活性氧（ROS）水平的降低。下游胁迫响应基因，包括COR、rd 29 A和ERD 10，也在过表达LoERF 017的转基因植物中被激活。总之，LoERF 017基因的过表达可以直接或间接地诱导抗氧化酶系统和下游胁迫反应基因，从而增强植物的抗渗透能力。
Dehydration-responsive element binding (DREB) transcription factors (TFs) play a significant role in the response to many abiotic stresses, and they can regulate the downstream expression of some defense genes. In this study, a novel DREB TF gene, designated as LoERF017, was identified from Larix olgensis. The ORF of the LoERF017 gene is 624 bp and it contains an AP2/EREBP domain belonging to a typical DREB TF. Sub-cellular location analysis showed that LoERF017 was located in the nucleus. QRT-PCR analysis indicated that LoERF017 could be induced by NaCl, PEG6000, sodium nitroprusside (SNP), and ABA treatment. Moreover, an analysis of the survival rate and the development of transgenic plants under mannitol and NaCl revealed that the overexpression of LoERF017 in Arabidopsis could improve tolerance to osmotic and salt stress. Compared with wild-type (WT) plants, the overexpression of LoERF017 resulted in higher superoxide dismutase (SOD) and peroxidase (POD) activities and lower malondialdehyde (MDA) and reactive oxygen species (ROS) levels under osmotic stress. Downstream stress-responsive genes, including COR, rd29A, and ERD10, were also activated in transgenic plants overexpressing LoERF017. In brief, the overexpression of the LoERF017 gene may directly or indirectly induce antioxidant enzyme systems and downstream stress-response genes to enhance osmotic resistance capacity in plants.