Developmental regulation of claudin localization by fetal alveolar epithelial cells
Developmental regulation of claudin localization by fetal alveolar epithelial cells
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DOI：
10.1152/ajplung.00423.2003
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发表时间：
2004-12-01
影响因子：
4.9
通讯作者：
Koval, M
中科院分区：
文献类型：
--
作者：
Daugherty, BL;Mateescu, M;Koval, M
紧密连接蛋白家族中的紧密连接蛋白调节上皮屏障功能。我们研究了在含有地塞米松、8-溴-cAMP和异丁基甲基黄嘌呤（DCI）的培养基中培养的人胎肺（HFL）肺泡上皮细胞的claudin表达，所述DCI促进肺泡上皮细胞分化为II型表型。在蛋白质水平，HFL细胞表达密蛋白-1、密蛋白-3、密蛋白-4、密蛋白-5、密蛋白-7和密蛋白-18，其中表达水平随培养条件而变化。在可渗透的支持物上培养的经DCI处理的分化的HFL细胞形成紧密的跨上皮屏障，跨上皮电阻（TER）> 1，700 ohm/cm（2）。相反，在没有DCI的对照培养基中培养的HFL细胞不形成紧密屏障（TER
Tight junction proteins in the claudin family regulate epithelial barrier function. We examined claudin expression by human fetal lung (HFL) alveolar epithelial cells cultured in medium containing dexamethasone, 8-bromo-cAMP, and isobutylmethylxanthanine (DCI), which promotes alveolar epithelial cell differentiation to a type II phenotype. At the protein level, HFL cells expressed claudin-1, claudin-3, claudin-4, claudin-5, claudin-7, and claudin-18, where levels of expression varied with culture conditions. DCI-treated differentiated HFL cells cultured on permeable supports formed tight transepithelial barriers, with transepithelial resistance ( TER)>1,700 ohm/cm(2). In contrast, HFL cells cultured in control medium without DCI did not form tight barriers (TER