Fibulin-4 deposition requires EMILIN-1 in the extracellular matrix of osteoblasts.
Fibulin-4 deposition requires EMILIN-1 in the extracellular matrix of osteoblasts.
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DOI：
10.1038/s41598-017-05835-7
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发表时间：
2017-07-17
影响因子：
4.6
通讯作者：
Sengle G
中科院分区：
文献类型：
--
作者：
Schiavinato A;Keene DR;Imhof T;Doliana R;Sasaki T;Sengle G
细胞外基质网络形成的组织微环境在调节组织结构和功能中起着重要作用。在这方面，由原纤维蛋白及其相关配体（如LTBP、纤蛋白和EMILIN）组成的细胞外微纤维网络特别令人感兴趣，因为它们提供了引导特定细胞类型的适当形态和功能行为的特定细胞微环境。要了解复杂的微纤维网络组成的细胞微环境如何影响细胞命运的决定，在上下文的方式，需要更多的信息，时空定位，沉积和功能的组件。通过采用共聚焦免疫荧光和电子显微镜，我们研究了EMILIN-1和-2在骨骼系统组织如软骨和骨以及成骨细胞体外培养物中的定位和细胞外基质沉积。我们发现，在RNAi介导的原代颅骨成骨细胞和MC 3 T3-E1细胞中EMILIN-1的消耗后，仅fibulin-4基质沉积丢失，而其他fibulin家族成员或LTBP保持不受影响。免疫沉淀和ELISA式结合试验证实了EMILIN-1和fibulin-4之间的直接相互作用。我们的数据表明，EMILIN-1的一个新功能，这意味着线性fibulin-4基质沉积的指导，从而fibulin-4纤维的形成。
Tissue microenvironments formed by extracellular matrix networks play an important role in regulating tissue structure and function. Extracellular microfibrillar networks composed of fibrillins and their associated ligands such as LTBPs, fibulins, and EMILINs are of particular interest in this regard since they provide a specialized cellular microenvironment guiding proper morphology and functional behavior of specialized cell types. To understand how cellular microenvironments composed of intricate microfibrillar networks influence cell fate decisions in a contextual manner, more information about the spatiotemporal localization, deposition, and function of their components is required. By employing confocal immunofluorescence and electron microscopy we investigated the localization and extracellular matrix deposition of EMILIN-1 and -2 in tissues of the skeletal system such as cartilage and bone as well as in in vitro cultures of osteoblasts. We found that upon RNAi mediated depletion of EMILIN-1 in primary calvarial osteoblasts and MC3T3-E1 cells only fibulin-4 matrix deposition was lost while other fibulin family members or LTBPs remained unaffected. Immunoprecipitation and ELISA-style binding assays confirmed a direct interaction between EMILIN-1 and fibulin-4. Our data suggest a new function for EMILIN-1 which implies the guidance of linear fibulin-4 matrix deposition and thereby fibulin-4 fiber formation.
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DOI：
10.1073/pnas.0908268106
发表时间：
2009-11-10
影响因子：
11.1
作者：
Horiguchi, Masahito;Inoue, Tadashi;Nakamura, Tomoyuki
通讯作者：
Nakamura, Tomoyuki
影响因子：
4.3
作者：
Bultmann-Mellin I;Conradi A;Maul AC;Dinger K;Wempe F;Wohl AP;Imhof T;Wunderlich FT;Bunck AC;Nakamura T;Koli K;Bloch W;Ghanem A;Heinz A;von Melchner H;Sengle G;Sterner-Kock A
通讯作者：
Sterner-Kock A
影响因子：
4.6
作者：
Corallo, Diana;Schiavinato, Alvise;Bonaldo, Paolo
通讯作者：
Bonaldo, Paolo
DOI：
10.1083/jcb.201008013
发表时间：
2011-10-03
期刊：
The Journal of cell biology
影响因子：
--
作者：
Danussi C;Petrucco A;Wassermann B;Pivetta E;Modica TM;Del Bel Belluz L;Colombatti A;Spessotto P
通讯作者：
Spessotto P
影响因子：
5.3
作者：
McLaughlin, PJ;Chen, QY;Marmorstein, LY
通讯作者：
Marmorstein, LY