Green tea polyphenol (-)-epigallocatechin-3-gallate treatment of human skin inhibits ultraviolet radiation-induced oxidative stress
Green tea polyphenol (-)-epigallocatechin-3-gallate treatment of human skin inhibits ultraviolet radiation-induced oxidative stress
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DOI：
10.1093/carcin/22.2.287
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发表时间：
2001-02-01
期刊：
影响因子：
4.7
通讯作者：
Mukhtar, H
中科院分区：
文献类型：
--
作者：
Katiyar, SK;Afaq, F;Mukhtar, H
使用天然存在的具有大量抗氧化活性的植物来保护人类皮肤免受紫外线损伤正受到越来越多的关注。绿色茶成分（-）-表没食子儿茶素-3-没食子酸酯（EGCG）是一种有效的抗氧化剂，并且在小鼠模型中显示出显著的预防光致癌和光毒性的效果。在本研究中，我们研究了局部应用EGCG的效果，存在于绿色茶中的主要多酚，对紫外线照射前人体皮肤上的紫外线诱导的氧化应激和抗氧化酶的标记物，使用免疫组织化学和分析酶测定，我们发现应用EGCG（类似于1 mg/cm（2）皮肤），在单次紫外线暴露4倍最小红斑剂量（MED）之前，显著降低紫外线诱导的过氧化氢产生表皮和真皮中NO（68- 90%，P < 0.025-0.005）和NO（30- 100%，P < 0.025-0.005）含量均呈时间依赖性。EGCG预处理还抑制UV诱导的炎性白细胞，特别是CD 11b（+）细胞（单核细胞/巨噬细胞和中性粒细胞的表面标志物）向皮肤中的浸润，其被认为是活性氧的主要产生者。EGCG处理还发现在所研究的每个时间点抑制UV诱导的表皮脂质过氧化（41- 84%，P < 0.05）。在研究的不同时间点，发现对人体皮肤进行4x MED的单次UV暴露可增加过氧化氢酶活性（109-145%），降低谷胱甘肽过氧化物酶（GPx）活性（36-54%）和总谷胱甘肽（GSH）水平（13-36%）。发现用EGCG预处理可恢复UV诱导的GSH水平下降，并对抗氧化酶GPx提供保护。需要进一步研究EGCG对人体皮肤多次暴露于紫外线的预防作用。
The use of naturally occurring botanicals with substantial antioxidant activity to afford protection to human skin against UV damage is receiving increasing attention, The green tea constituent (-)-epigallocatechin-3-gallate (EGCG) is a potent antioxidant and has shown remarkable preventive effects against photocarcinogenesis and phototoxicity in mouse models, In this study we have investigated the effects of topical application of EGCG, the major polyphenol present in green tea, to human skin before UV irradiation on UV-induced markers of oxidative stress and antioxidant enzymes, Using immunohistochemistry and analytical enzyme assays, we found that application of EGCG (similar to1 mg/cm(2) skin) before a single UV exposure of 4x minimal erythema dose (MED) markedly decreases UV-induced production of hydrogen peroxide (68-90%, P < 0.025-0.005) and nitric oxide (30-100%, P < 0.025-0.005) in both epidermis and dermis in a time-dependent manner. EGCG pretreatment also inhibits UV-induced infiltration of inflammatory leukocytes, particularly CD11b(+) cells (a surface marker of monocytes/macrophages and neutrophils), into the skin, which are considered to be the major producers of reactive oxygen species. EGCG treatment was also found to inhibit UV-induced epidermal lipid peroxidation at each time point studied (41-84%, P < 0.05). A single UV exposure of 4x MED to human skin was found to increase catalase activity (109-145%) and decrease glutathione peroxidase (GPx) activity (36-54%) and total glutathione (GSH) level (13-36%) at different time points studied. Pretreatment with EGCG was found to restore the UV-induced decrease in GSH level and afforded protection to the antioxidant enzyme GPx. Further studies are warranted to study the preventive effects of EGCG against multiple exposures to UV light of human skin.