Fos-like immunoreactivity in the brain of homozygous diabetes insipidus Brattleboro and normal Long-Evans rats.
Fos-like immunoreactivity in the brain of homozygous diabetes insipidus Brattleboro and normal Long-Evans rats.
复制标题
纯合性尿崩症 Brattleboro 大鼠和正常 Long-Evans 大鼠大脑中的 Fos 样免疫反应性。
DOI：
10.1002/cne.903220310
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发表时间：
1992
期刊：
影响因子：
--
通讯作者：
McCabe,JT
中科院分区：
文献类型：
--
作者：
Guldenaar,SE;Noctor,SC;McCabe,JT
缺水会诱导神经垂体系统神经元产生转录因子 Fos。这些神经元主要位于下丘脑室旁核 (PVN) 和视上核 (SON)，产生抗利尿激素加压素。目前的免疫细胞化学研究分析了 Fos 在参与渗透调节的大脑区域的分布，并比较了加压素缺乏的 Brattleboro 和正常 Long-Evans 大鼠在刺激和非刺激条件下的 Fos 免疫反应性 (Fos-IR) 程度。通过单次腹膜内注射 1.5 M/L NaCl 对大鼠进行渗透挑战。由于 Fos 可能是由处理动物的压力引起的，因此使用未注射和注射等渗盐水的大鼠作为对照。在未注射和注射等渗盐水的 Brattleboro 大鼠的终板血管器 (OVLT)、正中视前核 (MnPO)、穹窿下器官 (SFO) 和 SON 中发现了微弱的核 Fos 免疫染色，但在 Long-Evans 大鼠中未发现。 Long Evans 和 Brattleboro 大鼠中，高渗盐水注射特异性诱导位于 SFO、OVLT、MnPO、PVN、SON、下丘脑副核（包括环核）和下丘脑弓形核 (Arc) 的神经元中的 Fos-IR。菌株之间诱导的免疫染色的分布没有发现差异。处理和（等渗盐水）注射应激在外侧间隔核、中央杏仁核、内侧杏仁核、内侧视前区、终纹床核、扣带皮层和梨状皮层、外侧下丘脑区、下丘脑腹内侧核和缰核中诱导 Fos-IR 核。这些数据与 Fos 在急性高渗刺激期间调节加压素基因表达中的作用一致。此外，这项研究表明，在纯合 Brattleboro 大鼠经历的慢性渗透刺激期间，Fos-IR 有限，但显然持续存在，并且在急性渗透挑战后，Fos-IR 在这些动物中增加。我们的观察表明，纯合子 Brattleboro 大鼠的 c-fos 调节控制与 Long-Evans 大鼠的不同。 © 1992 Wiley-Liss, Inc.
Water deprivation induces the production of the transcription factor Fos in neurons of the neurohypophysial system. These neurons, which are located primarily in the hypothalamic paraventricular (PVN) and supraoptic nuclei (SON), produce the antidiuretic hormone vasopressin. The present immunocytochemical study has analyzed the distribution of Fos in brain regions involved in osmoregulation and compared the extent of Fos immunoreactivity (Fos‐IR) in vasopressin‐deficient Brattleboro and normal Long‐Evans rats under stimulated and non‐stimulated conditions. Rats were osmotically challenged by means of a single intraperitoneal injection of 1.5 M/L NaCl. Since Fos may be induced by the stress of handling of animals, non‐injected and isotonic saline‐injected rats were used as controls. Faint nuclear Fos immunostaining was found in the organum vasculosum of the lamina terminalis (OVLT), the median preoptic nucleus (MnPO), subfornical organ (SFO), and SON of non‐injected and isotonic saline‐injected Brattleboro but not Long‐Evans rats. Hypertonic saline injection specifically induced Fos‐IR in neurons located in the SFO, OVLT, MnPO, PVN, SON, hypothalamic accessory nuclei (including the nucleus circularis), and arcuate hypothalamic nucleus (Arc) in both Long Evans and Brattleboro rats. No differences in distribution of the induced immunostaining were found between the strains. Stress of handling and (isotonic saline) injection induced Fos‐IR in the lateral septal nuclei, central amygdaloid nuclei, medial amygdaloid nucleus, medial preoptic area, the bed nucleus of the stria terminalis, cingulate‐ and piriform cortex, the lateral hypothalamic area, ventromedial hypothalamic nucleus, and the habenular nucleus. The data are consistent with a role for Fos in the regulation of vasopressin gene expression during acute hyperosmotic stimulation. In addition, this study demonstrated that during chronic osmotic stimulation, as experienced by homozygous Brattleboro rats, Fos‐IR is limited but apparently present constantly and that it increased in these animals following acute osmotic challenge. Our observations suggest thatc‐fosregulatory controls in homozygous Brattleboro rats are different from those in Long‐Evans rats. © 1992 Wiley‐Liss, Inc.