CELLULAR STAGES IN CARTILAGE FORMATION AS REVEALED BY MORPHOMETRY, AUTORADIOGRAPHY AND TYPE-II COLLAGEN IMMUNOSTAINING OF THE MANDIBULAR CONDYLE FROM WEANLING RATS
CELLULAR STAGES IN CARTILAGE FORMATION AS REVEALED BY MORPHOMETRY, AUTORADIOGRAPHY AND TYPE-II COLLAGEN IMMUNOSTAINING OF THE MANDIBULAR CONDYLE FROM WEANLING RATS
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DOI：
10.1002/aja.1001820302
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发表时间：
1988-07-01
影响因子：
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通讯作者：
VONDERMARK, K
中科院分区：
文献类型：
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作者：
LUDER, HU;LEBLOND, CP;VONDERMARK, K
本研究应用形态计量学、~ 3 H-胸腺嘧啶核苷放射自显影、~ 3 H-脯氨酸放射自显影和软骨特异性Ⅱ型胶原免疫组化等方法，观察了细胞的增加、细胞的增大和基质的沉积在断乳大鼠髁突软骨内生长中的作用。从关节面向下，髁状突可分为五层，由嵌入基质中的细胞组成：1）关节层，由富含被认为是I型胶原的纤维的基质中的静止细胞组成，2）多形细胞层，包括祖细胞，经历软骨内变化的细胞从祖细胞产生，3）扁平细胞层，其中细胞产生缺乏II型胶原的前软骨基质，同时经历两个阶段的分化：“成软骨细胞”阶段和短的“扁平软骨细胞”：当细胞内II型胶原蛋白再生开始时的阶段，4）上部肥大细胞层，其中细胞是“典型的软骨细胞”，其以快速的速率扩大，积极地产生II型胶原蛋白，并将其存款到软骨基质中，和5）下部肥大细胞层，由处于终末扩大阶段的软骨细胞组成，同时软骨基质适应矿化。3 H-胸苷放射自显影结果表明，多态细胞层中祖细胞的更替时间约为2.9天。成软骨细胞在每个发育阶段所需的时间估计为1.4天，扁平软骨细胞为0.5天，上肥大细胞层的软骨细胞为2.3天，下肥大细胞层的软骨细胞为1.1天。计算参考1 ×从关节面延伸至血管浸润区的1 mm方形柱提供了细胞增加（0.0077 mm 3）、细胞外基质沉积（0.0127 mm 3）和细胞增大（0.0302 mm 3）的每日速率。因此，这三个因素对髁突生长的各自贡献的比例约为1：1.6：4。这一结果强调了细胞增大在髁突整体生长中所起的作用。
The role played by cell addition, cell enlargement, and matrix deposition in the endochondral growth of the condyle was assessed in weanling rats by four approaches makinguse of the light microscope: morphometry, 3H-thymidine radioautography, 3H-proline radioautography, and immunostaining for the cartilage-specific type II collagen. From the articular surface down, the condyle may be divided into five layers made up of cells embedded in a matrix: 1) the articular layter composed of static cells in a matrix rich in fibers presumed to be of type I collagen, 2) the polymorphic cell layer including the progenitor cell from which arise the cells undergoing endochondral changes, 3) the flattened cell layer in which cells produce a precartilagenous matrix devoid of type II collagen while undergoing differentiation in two stages: a "chondroblast" stage and a short "flattened chondrocyte: stage when intracellular type II collagen elabration begins, 4) the upper hypertrophic cell layer, in which cells are "typical chondrocytes" that enlarge at a rapid rate, actively produce type II collagen, and deposit it into a cartilagenous matrix, and 5) the lower hypertrophic cell layer, composed of chondrocytes at a stage of terminal enlargment while the cartilagenous matrix is adapting for mineralization. 3H-thymidine radioautographic results indicate that the turnover time of progenitor cells in the polymorphic cell layer is about 2.9 days. The time spent by cells at each stage of development is estimated to be 1.4 days as chrondroblasts, 0.5 days as flattened chrondrocytes, 2.3 days as the chondrocytes of the upper hypertropic cell layer, and 1.1day as those of the lower hypertrophic cell layer. Calculations referring to a 1 .times. 1-mm square-sided column extending from the articular surface to the zone of vascular invasion provide the daily rate of cell addition (0.0077 mm3), extracellular matrix deposition (0.0127 mm3) and cell enlargement (0.0302 mm3). Hence the respective contribution of the three factors to condyle growth is in a ratio of about 1:1.6:4. This result emphasizes the role played by cell enlargement in the overall growth of the condyle.