Establishment and maintenance of human embryonic stem cells on STO, a permanently growing cell line
Establishment and maintenance of human embryonic stem cells on STO, a permanently growing cell line
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DOI：
10.1095/biolreprod.103.017467
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发表时间：
2003-12-01
影响因子：
3.6
通讯作者：
Yoon, HS
中科院分区：
文献类型：
--
作者：
Park, JH;Kim, SJ;Yoon, HS
人类胚胎干细胞（hES）传统上是在小鼠原代胚胎成纤维细胞（pmef）上培养的。然而，尽管STO细胞作为供体细胞比pmef和人胚胎成纤维细胞（hef）有一些优势，但它们从未被用作供体细胞来建立hES细胞系。本研究以STO为喂细胞，从内细胞团（ICM）中建立了3株hES细胞系（mz - hes1、mz - hes2和mz - hes3）。3种he细胞系核型正常，碱性磷酸酶（AP）、细胞表面标记物（SSEA-3、SSEA-4、TRA-1-60和TRA-1-81）和转录因子Oct-4表达水平均较高。在STO细胞上培养2年后，he细胞保持了形成所有三种胚胎胚层衍生物的潜力。我们的研究结果表明，STO饲养细胞具有支持hES细胞系建立和维持的潜力。此外，我们的研究结果表明，层粘连蛋白可能在维持hES细胞的未分化增殖中发挥重要作用。
Human embryonic stem (hES) cells have been traditionally cultured on primary mouse embryonic fibroblasts (PMEFs). However, though STO cells have some advantages over PMEFs and human embryonic fibroblasts (hEFs) as feeder cells, they have never been used as feeder cells to establish hES cell lines. In this study, three hES cell lines (Miz-hES1, Miz-hES2, and Miz-hES3) were established from inner cell masses (ICM), using STO as feeder cells. The three hES cell lines had normal karyotypes and expressed high levels of alkaline phosphatase (AP), cell surface markers (SSEA-3, SSEA-4, TRA-1-60, and TRA-1-81), and transcription factor Oct-4. After culture on STO cells for 2 yr, hES cells maintained the potential to form derivatives of all three embryonic germ layers. Our results show that STO feeder cells have the potential to support the establishment and maintenance of hES cell lines. In addition, our results suggest that laminin may play an important role in maintaining the undifferentiated proliferation of hES cells.