THE SIMULTANEOUS EXTRACTION OF HIGH-MOLECULAR-WEIGHT DNA AND OF RNA FROM SOLID TUMORS
THE SIMULTANEOUS EXTRACTION OF HIGH-MOLECULAR-WEIGHT DNA AND OF RNA FROM SOLID TUMORS
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DOI：
10.1016/0003-2697(83)90299-3
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发表时间：
1983-01-01
影响因子：
2.9
通讯作者：
SAUER, G
中科院分区：
文献类型：
--
作者：
KRIEG, P;AMTMANN, E;SAUER, G
本文描述了一种从固体组织中分离大分子DNA和RNA的新方法，该方法基于在称为Mikro-dismembrane的机械装置中通过振动破坏深度冷冻的材料。该技术揭示了平均1-3 mg DNA或RNA/g组织的产量。纯化核酸的质量允许对整合的肿瘤病毒DNA序列及其mRNA转录物进行详细分析。从角化疣组织中有效分离乳头状瘤病毒粒子是通过应用Mikro-dismembrane来促进的。
A novel method for the isolation of both macromolecular DNA and RNA from solid tissues based on the disruption by vibration of deep-frozen material in a mechanical device termed Mikro-dismembrator, is described. This technique reveals a yield of, on the average, 1-3 mg of either DNA or RNA/g of tissue. The quality of the purified nucleic acids permits the detailed analysis of integrated tumor virus DNA sequences and their mRNA transcripts. The efficient isolation of papilloma virions from keratinized wart tissue is facilitated by the application of the Mikro-dismembrator.