Proteomic approach:: Identification of Medicago truncatula proteins induced in roots after infection with the pathogenic oomycete Aphanomyces euteiches
Proteomic approach:: Identification of Medicago truncatula proteins induced in roots after infection with the pathogenic oomycete Aphanomyces euteiches
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DOI：
10.1007/s11103-004-0499-1
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发表时间：
2004-05-01
影响因子：
5.1
通讯作者：
Krajinski, F
中科院分区：
文献类型：
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作者：
Colditz, F;Nyamsuren, O;Krajinski, F
豆科植物根腐病是豆科作物生产中的主要减产因素之一。采用比较蛋白质组学方法，对模式豆科牧草紫花苜蓿感染真核白僵菌后的调控蛋白进行了鉴定。用双向凝胶电泳法鉴定了病原菌攻击后差异表达的几种蛋白质。在分析的时间过程中，表达值的密度计量学评估显示出不同的规律。用基质辅助激光解吸电离飞行时间质谱仪(MALDI-TOF-MS)鉴定感染过程中调节的蛋白质。在差异表达的蛋白质中，两个编码细胞壁蛋白，两个被命名为小分子热休克蛋白。此外，查尔酮-O-甲基转移酶的一个亚型在受感染的根中也增加了。大多数诱导蛋白属于致病相关蛋白(PR10)10类家族。以前，各种类似PR10的蛋白已被证明受一般应激或脱落酸(ABA)的调节。因此，我们进一步研究了这些蛋白质在干旱胁迫和外源ABA作用下的调控作用。确定了复杂的调控模式：外源ABA也诱导了3个红曲霉诱导的类PR10蛋白，但没有一个在干旱胁迫后诱导。相比之下，其中三种蛋白质在干旱胁迫下表达下调。因此，不同的PR10家族成员及其调控谱的强烈表达表明，这一组蛋白在根对各种胁迫条件的适应过程中发挥了重要作用。
The legume root rot disease caused by the oomycete pathogen Aphanomyces euteiches is one major yield reducing factor in legume crop production. A comparative proteomic approach was carried out in order to identify proteins of the model legume Medicago truncatula which are regulated after an infection with A. euteiches. Several proteins were identified by two dimensional gel electrophoresis to be differentially expressed after pathogen challenge. Densitometric evaluation of expression values showed different regulation during the time-course analysed. Proteins regulated during the infection were identified by matrix assisted laser desorption/ionization-time of flight-mass spectrometry (MALDI-TOF-MS). Among the differentially expressed proteins, two encoded putative cell wall proteins and two were designated as small heat shock proteins. Furthermore, an isoform of the chalcone-O-methyltransferase was found to be increased in infected roots. The majority of induced proteins belonged to the family of class 10 of pathogenesis related proteins (PR10). Previously, various PR10-like proteins have been shown to be regulated by general stress or abscisic acid (ABA). Therefore, these proteins were further investigated concerning their regulation in response to drought stress and exogenous ABA-application. Complex regulation patterns were identified: three of the A. euteiches-induced PR10-like proteins were also induced by exogenous ABA-but none of them is induced after drought stress. In contrast, three of these proteins are down-regulated by drought stress. Hence, the strong expression of different PR10-family members and their regulation profiles indicates that this set of proteins plays a major role during root adaptations to various stress conditions.