Inhibition of Rho-kinase protects the heart against ischemia/reperfusion injury
Inhibition of Rho-kinase protects the heart against ischemia/reperfusion injury
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DOI：
10.1016/j.cardiores.2003.12.004
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发表时间：
2004-02-15
影响因子：
10.8
通讯作者：
Yue, TL
中科院分区：
文献类型：
--
作者：
Bao, WK;Hu, E;Yue, TL
目的：探讨Rho A和Rho-K在急性心肌缺血/再灌注损伤中的作用及Rho-K抑制剂Y-27632[(R)-(+)-trans-N-(4-pyridyl)-4-(1-aminoethyl)cyclohexanecarboxamide].的保护作用方法和结果：雄性CD1小鼠冠脉结扎30min，再灌注2 4h。缺血/再灌注可上调缺血心肌中Rho A的表达，进而激活Rho-Kinase。Y-27632显著抑制缺血/再灌流后Rho-Kinase的激活。Y-27632 10 mg/kg和30 mg/kg灌胃给药后，心肌梗死面积分别缩小30.2%和41.1%(P
Objective: To investigate the role of Rho A and Rho-kinase in acute myocardial ischemia/reperfusion injury and the protective effect of Rho-kinase inhibitor, Y-27632 [(R)-(+)-trans-N-(4-pyridyl)-4-(1-aminoethyl)cyclohexanecarboxamide]. Methods and results: Male CD1 mice were subjected to 30 min of coronary occlusion and 24 h reperfusion. Ischemia/reperfusion upregulated expression of Rho A in ischemic myocardium, and subsequently activated Rho-kinase. Y-27632 significantly inhibited the activation of Rho-kinase following ischemia/reperfusion. Treatment with Y-27632 at 10 and 30 mg/kg oral administration, reduced infarct size by 30.2% and 41.1%, respectively (P