17α-Hydroxyprogesterone, 4-Androstenedione, and Testosterone Profiled by Routine Stable Isotope Dilution/Gas Chromatography-Mass Spectrometry in Plasma of Children
17α-Hydroxyprogesterone, 4-Androstenedione, and Testosterone Profiled by Routine Stable Isotope Dilution/Gas Chromatography-Mass Spectrometry in Plasma of Children
复制标题
通过常规稳定同位素稀释/气相色谱-质谱法分析儿童血浆中的 17α-羟基孕酮、4-雄烯二酮和睾酮
作者：
S. Wudy;U. Wachter;J. Homoki;W. Teller
摘要：采用稳定同位素稀释/气相色谱-质谱（ID/GC-MS），一种物理化学方法，我们分析了不同年龄组正常儿童血浆类固醇17α-羟孕酮、4-雄烯二酮和睾酮。我们的值与直接免疫测定和使用提取或纯化步骤获得的值的比较表明，免疫测定一般高估类固醇浓度。对于新生儿期的血浆样本尤其如此，并且在17α-羟孕酮浓度中表现得最明显。我们的研究证明了ID/GC-MS对常规临床类固醇分析的适用性。当可能出现基质效应或交叉反应性问题或需要重新检查免疫测定的可疑结果时，建议应用ID/GC-MS。
ABSTRACT: Using stable isotope dilution/gas chromatography-mass spectrometry (ID/GC-MS), a physicochemical method, we have profiled the plasma steroids 17α-hydroxyprogesterone, 4-androstenedione, and testosterone in normal children of various age groups. Comparison of our values with those obtained by direct immunologic assays and those using an extraction or purification step showed that immunoassays in general overestimate steroid concentrations. This was especially true for plasma samples in the neonatal period and was most expressed for the concentrations of 17α-hydroxyprogesterone. Our study demonstrated the applicability of ID/GC-MS to routine clinical steroid analysis. The application of ID/GC-MS is recommended whenever problems from matrix effects or cross-reactivity are likely to arise or suspicious results by immunoassays need to be rechecked.