Simultaneous acetone-butanol-ethanol fermentation, gas stripping, and full-cell-catalyzed esterification for effective production of butyl oleate
Simultaneous acetone-butanol-ethanol fermentation, gas stripping, and full-cell-catalyzed esterification for effective production of butyl oleate
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同时丙酮-丁醇-乙醇发酵、气提和全细胞催化酯化有效生产油酸丁酯
DOI：
10.1007/s00449-018-1960-x
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发表时间：
2018-09-01
影响因子：
3.8
通讯作者：
Tan, Tianwei
中科院分区：
文献类型：
--
作者：
Chen, Changjing;Cai, Di;Tan, Tianwei
为了提高丙酮-丁醇-乙醇（ABE）发酵工艺的经济可行性，获得有价值的产品，并延伸产品链，本研究将Candida sp. 99-125细胞催化酯化与ABE发酵-气提集成系统相结合。含有浓缩ABE产物的气提冷凝液直接用于酯化反应，而不需要有毒有机溶剂的参与。考察了酯化反应中全细胞催化温度和细胞投加速率对油酸酯生成的影响。在优化条件下（35 ℃，8%细胞），酯化4 h后，油酸丁酯和油酸乙酯的收率分别为68%和12%。念珠菌属99-125细胞能够重复使用至少四个循环。该工艺不仅具有良好的环境效益，而且在提高传统ABE发酵工艺的经济可行性方面也具有广阔的应用前景。
In this study, aiming to improve the economic feasibility of acetone-butanol-ethanol (ABE) fermentation process, generate valuable products and extend the product chain, esterification catalyzed by Candida sp. 99-125 cells was hybrid with the ABE fermentation-gas-stripping integration system. The gas-stripping condensate that contained concentrated ABE products was directly used for esterification without the participation of toxic organic solvents. Full-cell catalysis temperature and the cell dosage rate on oleate production were evaluated and optimized in the esterification process. Under the optimized conditions (35 degrees C, 8% of cells), similar to 68% of butyl oleate and similar to 12% of ethyl oleate were obtained after 4 h of esterification. The Candida sp. 99-125 cells were able to be reused for at least four cycles. The novel cascade process showed environmental benefits, which also showed promising in improving the economic feasibility of the conventional ABE fermentation process.