Chronic stress associated with spousal caregiving of patients with Alzheimer's dementia is associated with downregulation of B-lymphocyte GH mRNA.
Chronic stress associated with spousal caregiving of patients with Alzheimer's dementia is associated with downregulation of B-lymphocyte GH mRNA.
复制标题
与阿尔茨海默氏痴呆患者的配偶照顾相关的慢性压力与 B 淋巴细胞 GH mRNA 的下调有关。
DOI：
10.1093/gerona/54.4.m212
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发表时间：
1999
期刊：
影响因子：
--
通讯作者：
Malarkey,WB
中科院分区：
文献类型：
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作者：
Wu,H;Wang,J;Cacioppo,JT;Glaser,R;Kiecolt-Glaser,JK;Malarkey,WB
研究背景：生长激素（GH）是由人外周血单个核细胞（PBMC）合成和分泌的，GH mRNA的表达遍及整个人体免疫系统。我们利用定量RT-PCR来确定T-和B-细胞群体中的GH mRNA水平从PBMC。受试者是9个照顾者的AD患者和9个年龄和性别匹配的controls.Results.In对照组，我们发现一个三倍以上的GH mRNA的表达在B细胞比在T细胞。这一发现与我们以前的原位杂交观察结果一致，表明GH mRNA主要存在于人类免疫器官的B细胞区域。我们还发现，从总的外周血单个核细胞和B细胞中GH mRNA的表达在照顾者中分别比对照组低50%和60%。我们的研究结果表明，B-细胞生长激素mRNA可能有助于对流感病毒疫苗接种的免疫应答较差，这在以前的慢性应激照顾者中已有报道。
Background.It has been demonstrated that growth hormone (GH) is synthesized and secreted by human peripheral mononuclear cells (PBMC), and the expression of GH mRNA can be found throughout the human immune system.Methods.We studied a population of female caregivers of patients with Alzheimer's dementia (AD) who suffered from the stress of caring for these patients. We utilized quantitative RT-PCR to determine GH mRNA levels in T- and B-cell populations from PBMC. Subjects were nine caregivers of AD patients and nine age- and sex-matched controls.Results.In the control group we found a threefold greater GH mRNA expression in B cells than in T cells. This finding was consistent with our previous in situ hybridization observation, suggesting GH mRNA in predominately B-cell areas of immune organs in humans. We also found that the expression of GH mRNA from total peripheral blood mononuclear cells and B cells in caregivers was 50% and 60% respectively less than that in the control group.Conclusions.Because the B-cell population is the source of antibody production, our findings suggest that the decrease in B-cell GH mRNA may contribute to the poor immune response to influenza virus vaccination that has been reported previously in chronically stressed caregivers.