Metagenomic analysis reveals potential biodegradation pathways of persistent pesticides in freshwater and marine sediments
Metagenomic analysis reveals potential biodegradation pathways of persistent pesticides in freshwater and marine sediments
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宏基因组分析揭示了淡水和海洋沉积物中持久性农药的潜在生物降解途径
DOI：
10.1016/j.scitotenv.2013.10.076
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发表时间：
2014-02-01
影响因子：
9.8
通讯作者：
Zhang, Tong
中科院分区：
文献类型：
--
作者：
Fang, Hua;Cai, Lin;Zhang, Tong
采用宏基因组学方法，研究了6个数据集（16 Gb）中DDT、HCH和ATZ在淡水和海洋沉积物中的生物降解基因（BDGs）的丰度和多样性，以及它们的潜在降解途径。这些数据集是使用Illumina高通量测序获得的，并基于BLAST对自行建立的BDGs、DDT降解基因（DDGs）、HCH降解基因（HDGs）和ATZ降解基因（ADGs）数据库。结果表明，不同来源和不同地点的样品，BDGs、DDGs、HDGs和ADGs的丰度和多样性存在差异。检测到编码过氧化物酶的lip和mnp基因以及编码漆酶的carA基因为降解有机污染物的显性基因。hdt，hdg和atzB基因，编码水合酶，脱卤酶，和乙氨基水解酶，被认为是最丰富的基因参与滴滴涕，六六六，和ATZ的降解，分别。经鉴定，具有降解有机污染物能力的69个属中，变形菌门（49.3%）和放线菌门（21.7%）最多。四个属，包括邻囊藻，厌氧微生物，詹纳斯基亚，和分枝杆菌，是所有沉积物中的主要生物降解种群。在本研究中，DDT和ATZ几乎完全降解，而HCH在所有沉积物中都存在部分降解。(C)2013爱思唯尔有限公司版权所有。
The abundance and diversity of biodegradation genes (BDGs) and potential degradation pathways of dichlorodiphenyltrichloroethane (DDT), hexachlorocydohexane (HCH), and atrazine (ATZ) in freshwater and marine sediments were investigated by metagenomic analysis using 6 datasets (16 Gb in total). The datasets were derived using Illumina high-throughput sequencing and were based on BLAST against self-established databases of BDGs, DDT degradation genes (DDGs), HCH degradation genes (HDGs), and ATZ degradation genes (ADGs). The results showed that the abundance and diversity of BDGs, DDGs, HDGs, and ADGs varied with sample source and locations. The lip and mnp genes, which encode for peroxidase, and the carA gene, which encodes for laccase, were detected as the dominant genes for degradation of organic pollutants. The hdt, hdg, and atzB genes, which encode for hydratase, dehalogenase, and ethylaminohydrolase, were found to be the most abundant genes involved in DDT, HCH, and ATZ degradation, respectively. The identified 69 genera capable of degrading organic pollutants were mostly affiliated with Proteobacteria (49.3%) and Actinobacteria (21.7%). Four genera, including Plesiocystis, Anaerolinea, Jannaschia, and Mycobacterium, were the major biodegradation populations in all sediments. In this study, the nearly complete biodegradation pathways of DDT and ATZ were found, and the partial degradation pathway of HCH was detected in all sediments. (C) 2013 Elsevier B.V. All rights reserved.