Arabidopsis thaliana-derived resistance against Leptosphaeria maculans in a Brassica napus genomic background
Arabidopsis thaliana-derived resistance against Leptosphaeria maculans in a Brassica napus genomic background
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DOI：
10.1007/s00122-002-0885-5
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发表时间：
2002-09-01
影响因子：
5.4
通讯作者：
Dixelius, C
中科院分区：
文献类型：
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作者：
Bohman, S;Wang, M;Dixelius, C
甘蓝型油菜茎溃烂病是由斑点钩端螺旋体引起的一种广泛存在的病害。相反，大多数拟南芥材料是高度抗性的。因此，从甘蓝型油菜和拟南芥的对称和不对称体细胞杂种中获得的新材料被用于斑点落叶松抗性的筛选。最初，子叶和成体叶片的抗性性状都从拟南芥转移到甘蓝型油菜。在后代中，这两个性状分离，子叶抗性丧失。从基因组定位和蛋白质表达两个方面研究了成叶抗性。对抗性植株剩余DNA的分析表明，成虫叶片抗性与3号染色体分子标记之间存在共分离。对含有3号染色体片段的不对称杂交植物产生的抗性后代进行了更详细的研究。确定了位于9.8-10.4MBP和18-19.5MBP两个区域，其中有几个与防御相关的基因。蛋白质组学方法被用来进一步研究参与防御相互作用的基因。接种L斑点病菌48h后，尽管存在额外的拟南芥染色体，但只有乙醇酸氧化酶等少数蛋白质在抗病品系中与甘蓝型油菜有差异表达。本研究中描述的植物材料构成了一个新的抗斑点落叶松遗传来源，目前正被纳入甘蓝型油菜育种计划。
Stem canker (blackleg) caused by Leptosphaeria maculans is a widespread disease of Brassica napus. In contrast, most Arabidopsis thaliana accessions are highly resistant. Hence, novel material derived from symmetric and asymmetric somatic hybrids between B. napus and A. thaliana was utilised in a screen for L. maculans resistance. Initially, both cotyledon and adult-leaf resistance traits were transferred from A. thaliana to B. napus. In later generations the two traits segregated and cotyledon resistance was lost. The adult-leaf resistance was investigated with respect to genome localisation and protein expression. Analyses of remaining A. thaliana DNA in resistant plants showed co-segregation between adult-leaf resistance and chromosome-3 molecular markers. Resistant offspring from asymmetric hybrid plants that contained fragments of chromosome 3 were studied in more detail. Two regions at positions 9.8-10.4 Mbp and 18-19.5 Mbp, where several defence-related genes are located, were identified. A proteomic approach was taken to further investigate genes involved in the defence interaction. Forty eight hours after inoculation with L maculans, only a few proteins, such as glycolate oxidase, were identified as differentially expressed in the resistant line compared to B. napus, despite the presence of additional A. thaliana chromosomes. The plant materials described in the present study constitute a new genetic source of L. maculans resistance and are currently being incorporated into B. napus breeding programmes.