Central NPY-Y5 sub-receptor partially functions as a mediator of NPY-induced hypothermia and affords thermotolerance in heat-exposed fasted chicks.
Central NPY-Y5 sub-receptor partially functions as a mediator of NPY-induced hypothermia and affords thermotolerance in heat-exposed fasted chicks.
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DOI：
10.14814/phy2.13511
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发表时间：
2017-12
影响因子：
2.5
通讯作者：
Chowdhury VS
中科院分区：
文献类型：
--
作者：
Eltahan HM;Bahry MA;Yang H;Han G;Nguyen LTN;Ikeda H;Ali MN;Amber KA;Furuse M;Chowdhury VS
先前研究表明，雏鸡暴露在较高的环境温度(HT)下会增加神经肽Y(NPY)在大脑中的表达。此外，还发现NPY对热暴露禁食雏鸡具有抗应激作用。本研究旨在揭示中枢应用NPY对禁食雏鸡耐热能力的影响，以及血浆代谢产物变化对热休克蛋白(HSP)和NPY亚受体(NPYSRs)的诱导作用。6或7日龄雏鸡中央注射0或375pmoL的NPY，禁食时暴露于高温(35℃±31℃)或控制温度(CT：30℃±31℃)60分钟。NPY可降低CT和HT下的体温。在CT和HT条件下，NPY可增强HSP-70和-90以及NPYSRs-Y5、-Y6和-Y7的表达，但不能增加-Y1、-Y2和-Y4的表达。联合注射NPYSR-Y5拮抗剂(CGP71683)和NPY(375pmol)可减轻NPY引起的体温降低。此外，中枢NPY还能降低CT和HT时的血糖和三酰甘油，并使HT时的血浆皮质酮和肾上腺素水平降低。NPY可增加血浆牛磺酸和氨丝氨酸浓度。综上所述，脑NPYSR-Y5对热暴露禁食雏鸡具有部分保护性耐热性。NPY介导的血糖和应激激素水平的降低以及血浆游离氨基酸的增加进一步表明NPY可能在减少禁食雏鸡的热应激中发挥作用。
Exposure of chicks to a high ambient temperature (HT) has previously been shown to increase neuropeptide Y (NPY) mRNA expression in the brain. Furthermore, it was found that NPY has anti‐stress functions in heat‐exposed fasted chicks. The aim of the study was to reveal the role of central administration of NPY on thermotolerance ability and the induction of heat‐shock protein (HSP) and NPY sub‐receptors (NPYSRs) in fasted chicks with the contribution of plasma metabolite changes. Six‐ or seven‐day‐old chicks were centrally injected with 0 or 375 pmol of NPY and exposed to either HT (35 ± 1°C) or control thermoneutral temperature (CT: 30 ± 1°C) for 60 min while fasted. NPY reduced body temperature under both CT and HT. NPY enhanced the brain mRNA expression of HSP‐70 and ‐90, as well as of NPYSRs‐Y5, ‐Y6, and ‐Y7, but not ‐Y1, ‐Y2, and ‐Y4, under CT and HT. A coinjection of an NPYSR‐Y5 antagonist (CGP71683) and NPY (375 pmol) attenuated the NPY‐induced hypothermia. Furthermore, central NPY decreased plasma glucose and triacylglycerol under CT and HT and kept plasma corticosterone and epinephrine lower under HT. NPY increased plasma taurine and anserine concentrations. In conclusion, brain NPYSR‐Y5 partially afforded protective thermotolerance in heat‐exposed fasted chicks. The NPY‐mediated reduction in plasma glucose and stress hormone levels and the increase in free amino acids in plasma further suggest that NPY might potentially play a role in minimizing heat stress in fasted chicks.