Development of Cutaneous Bioadhesive Ureasil-Polyether Hybrid Films
Development of Cutaneous Bioadhesive Ureasil-Polyether Hybrid Films
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DOI：
10.1155/2015/727324
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发表时间：
2015-01-01
影响因子：
3.3
通讯作者：
Chiavacci, Leila Aparecida
中科院分区：
文献类型：
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作者：
Oshiro Junior, Joao Augusto;Carvalho, Flavia Chiva;Chiavacci, Leila Aparecida
尿硅醚-聚醚膜合成中涉及的水解和缩合反应影响膜形成时间和能够与生物基质形成氢键的化学基团的数量，负责生物粘附。本工作的目的是研究在水解和缩合反应中使用酸催化剂（盐酸）和碱性催化剂（氟化铵）对脲硅聚醚膜的形成时间和生物粘附性的影响。评价了膜的毒性。MTT测定显示，人皮肤角质形成细胞的细胞活力高于所有分析材料的70%，表明细胞毒性低。膜的生物粘附性强烈依赖于用于合成混合分子的聚醚链的粘度和亲水/疏水平衡。酸催化剂的使用促进了具有较高生物粘附性的较低粘性膜的形成。由更亲水的PEO链形成的杂化物更具有生物粘附性，因为它们可以更有效地与角质层中存在的水相互作用，从而增加生物粘附性。由于它们的低毒性和高生物粘附性，通过使用HCl作为催化剂获得的脲硅-PEO膜是作为生物粘附膜应用于皮肤的良好候选者。
The hydrolysis and condensation reactions involved in synthesis of ureasil-polyether films influence the film formation time and the number of chemical groups able to form hydrogen bonds, responsible for the bioadhesion, with the biological substrate. The objective of this work was to study the influence of the use of an acid catalyst (hydrochloric acid) and a basic catalyst (ammonium fluoride) in the hydrolysis and condensation reactions on the time formation and bioadhesion of ureasil-polyether films. The toxicity of the films was evaluated. The MTT assay has shown cell viability of human skin keratinocytes higher than 70% of all analyzed materials suggesting low cytotoxicity. The bioadhesion of the films is strongly dependent on the viscosity and hydrophilic/hydrophobic balance of the polyether chains used to synthetize the hybrid molecules. The use of acid catalyst promotes the formation of less viscous films with higher bioadhesion. The hybrids formed by more hydrophilic PEO chains are more bioadherent, since they can interact more efficiently with the water present in the stratum corneum increasing the bioadhesion. Due to their low toxicity and high bioadhesion, the ureasil-PEO films obtained by using HCl as catalyst agent are good candidates for application to the skin as bioadhesive films.