Benzyloxycarbonyl-Val-Ala-Asp (OMe) fluoromethylketone (Z-VAD.FMK) inhibits apoptosis by blocking the processing of CPP32
Benzyloxycarbonyl-Val-Ala-Asp (OMe) fluoromethylketone (Z-VAD.FMK) inhibits apoptosis by blocking the processing of CPP32
复制标题
DOI：
10.1042/bj3150021
复制
发表时间：
1996-04-01
影响因子：
4.1
通讯作者：
Cohen, GM
中科院分区：
文献类型：
--
作者：
Slee, EA;Zhu, HJ;Cohen, GM
白细胞介素-1 β转换酶（Interleukin-1 β converting enzyme， ICE）样蛋白酶作为无活性前体被合成，在诱导细胞凋亡中起关键作用。我们现在证明了苯氧羰基- val - ala - asp （OMe）氟甲基酮（Z-VAD）。FMK)是一种ice样蛋白酶抑制剂，通过阻止CPP32加工成其活性形式来抑制细胞凋亡。这些结果表明，可以开发新的细胞凋亡抑制剂来阻止ice样蛋白酶的加工形式。
Interleukin-1 beta converting enzyme (ICE)-like proteases, which are synthesized as inactive precursors, play a key role in the induction of apoptosis. We now demonstrate that benzyloxycarbonyl-Val-Ala-Asp (OMe) fluoromethylketone (Z-VAD.FMK), an ICE-like protease inhibitor, inhibits apoptosis by preventing the processing of CPP32 to its active form. These results suggest that novel inhibitors of apoptosis can be developed which prevent processing of proforms of ICE-like proteases.