Genetic differentiation of wild relatives of rice as assessed by RFLP analysis
Genetic differentiation of wild relatives of rice as assessed by RFLP analysis
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DOI：
10.1007/s00122-002-1013-2
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发表时间：
2002-10
影响因子：
5.4
通讯作者：
Bao-Rong Lu;K. Zheng;H. Qian;J. Zhuang
中科院分区：
文献类型：
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作者：
Bao-Rong Lu;K. Zheng;H. Qian;J. Zhuang
为研究水稻野生近缘种质资源的遗传多样性和亲缘关系，对58份水稻（Oryza rufipogon,Oryza nivara,Oryza sativaf）进行了分析。利用限制性片段长度多态性（RFLP）技术对分布广泛的天然稻和栽培稻进行了分析。所有使用的30个RFLP探针都检测到theoryzaacacina的多态性，每个探针平均检测到3.8个多态性片段。遗传多样性较高，相似系数在0.28 ~ 0.93之间；但聚类分析并未显示出明显的基于物种分类的类群，而是随机分散在不同的类群中。值得注意的是，来自同一地理区域或邻近地理区域的theoryzaacessio往往聚集在相同的组中。籼稻品种表现出较高的遗传多样性，分散在其野生近缘的不同类群中，而粳稻品种表现出相对较低的变异，形成了一个独立的类群。分子分析结果表明：(1)4个稻谷类群亲缘关系密切，其独立种地位有待进一步研究；(2)地理隔离对物种的分化起着重要作用；因此，野生稻种质资源的收集和保护需要覆盖广泛的地理范围；(3)传统的结论，即金菖蒲是从其祖先野生物种直接驯化而来，而日本金菖蒲是从金菖蒲衍生而来，这一结论得到了本研究数据的支持。
To study genetic diversity and relationships of wild relatives of rice, 58 accessions ofOryza rufipogon,Oryza nivara,Oryza sativaf.spontaneaand the cultivatedOryza sativa, representing a wide range of their distribution, were analyzed using the restriction fragment length polymorphism (RFLP) technique. All 30-used RFLP probes detected polymorphisms among theOryzaaccessions, with an average of 3.8 polymorphic fragments per probe. Considerable genetic diversity was scored among theOryzaaccessions with a similarity coefficient ranging from 0.28 to 0.93; but the cluster analysis of the accessions did not show an apparent grouping based on the species classification, instead they were scattered randomly in different groups. Noticeably, theOryzaaccessions from the same geographic region, or near-by geographic regions, tended to be clustered in the same groups. Theindicarice varieties showed relatively high genetic diversity and were scattered in different groups of their wild relatives, but thejaponicavarieties showed a relatively low variation and formed an independent group. It is concluded from the molecular analytical result that: (1) the fourOryzataxa have a remarkably close relationship and their independent species status need to be carefully reviewed; (2) geographic isolation has played a significant role in the differentiation of theOryzaaccessions; therefore, a wide geographic range needs to be covered in collecting wild rice germplasm forex situconservation; and (3) the conventional conclusion ofindicarice being directly domesticated from its ancestral wild species, andjaponicarice being derived fromindica, gains support from our data.