RNA CODONS AND PROTEIN SYNTHESIS .15. DISSIMILAR RESPONSES OF MAMMALIAN AND BACTERIAL TRANSFER RNA FRACTIONS TO MESSENGER RNA CODONS
RNA CODONS AND PROTEIN SYNTHESIS .15. DISSIMILAR RESPONSES OF MAMMALIAN AND BACTERIAL TRANSFER RNA FRACTIONS TO MESSENGER RNA CODONS
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DOI：
10.1016/0022-2836(68)90076-4
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发表时间：
1968-01-01
影响因子：
5.6
通讯作者：
NIRENBERG, M
中科院分区：
文献类型：
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作者：
CASKEY, CT;BEAUDET, A;NIRENBERG, M
来自豚鼠肝脏和大肠杆菌的未分级氨酰-tRNA制剂对大多数mRNA密码子的反应相似，但不是所有的。为了阐明不同的反应，肝和E。通过柱层析分离大肠杆菌氨酰-tRNA制备物，并测定三核苷二磷酸密码子对纯化的氨酰-tRNA组分与核糖体结合的影响。16种哺乳动物的氨酰-tRNA被发现对应于7个氨基酸。比较了六种氨基酸的密码子-反密码子关系。只有7种通用的氨酰-tRNA被发现与这两种酶。大肠杆菌和肝氨酰-tRNA。7种氨酰-tRNA从肝脏中分离得到，用E.大肠杆菌制备的氨基酰-tRNA;相反，弗罗姆中获得了5种氨基酰-tRNA。结果还表明，对于某些密码子，某些生物可能含有很少或不含氨酰-tRNA。例如，肝脏异亮氨酸-tRNA对AUA反应良好，而对E没有反应。大肠异亮氨酸-tRNA。肝脏中的精氨酸-tRNA对AGG反应良好，但对阿加反应不足;大肠杆菌tRNA对阿加和AGG的反应相对不足。E.大肠半胱氨酸-tRNA识别UGU、UGC和UGA;在相同条件下，肝半胱氨酸-tRNA对UGU和UGC有反应，但对UGA无反应。弗罗姆. coliCR 63，而不弗罗姆.大肠杆菌这些研究结果进行了讨论的tRNA抑制剂和可能的机制，调节蛋白质synthesization.Mammalian和细菌aminoacyl-tRNA馏分响应密码子组根据摆动碱基配对。另一种简并模式，其中A，G或U可能占据同义三联体的第三位置，观察到E的两个峰。大肠杆菌素-tRNA。
Unfractionated aminoacyl-tRNA preparations from guinea pig liver andEscherichia colirespond similarly to most, but not to all, mRNA codons. To clarify the dissimilar responses, liver andE. coliaminoacyl-tRNA preparations were fractionated by column chromatography, and the effects of trinucleoside diphosphate codons upon the binding of purified aminoacyl-tRNA fractions to ribosomes were determined. Sixteen species of mammalian aminoacyl-tRNA were found corresponding to seven amino acids. Codon-anticodon relationships for six amino acids were compared. Only seven universal species of aminoacyl-tRNA were found with bothE. coliand liver aminoacyl-tRNA. Seven species of aminoacyl-tRNA were obtained from liver that were not detected withE. colipreparations; conversely, five species of aminoacyl-tRNA were obtained fromE. colithat were not found with liver preparations.The results also suggest that some organisms may contain little or no aminoacyl-tRNA for certain codons. For example, liver isoleucine-tRNA responds well to AUA; no response was detected withE. coliisoleucine-tRNA. Liver arginine-tRNA responds well to AGG, but was deficient in response to AGA;E. coliarginine-tRNA was relatively deficient in response to AGA and AGG. One species ofE. colicysteine-tRNA recognizes UGU, UGC and UGA; under identical conditions liver cysteine-tRNA responds to UGU and UGC, but not to UGA. A species of serine-tRNA responding to UAG was obtained fromE. coliCR63, but not fromE. coliB. These findings are discussed in terms of tRNA suppressors and possible mechanisms for regulating protein synthesis.Mammalian and bacterial aminoacyl-tRNA fractions respond to sets of codons according to wobble base-pairing. Another pattern of degeneracy, in which A, G or U may occupy the third position of synonym triplets, was observed with two peaks ofE. coliserine-tRNA.