Foreign gene products can be enhanced by introduction into low storage protein mutants
Foreign gene products can be enhanced by introduction into low storage protein mutants
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DOI：
10.1046/j.1467-7652.2003.00038.x
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发表时间：
2003-11-01
影响因子：
13.8
通讯作者：
Takaiwa, F
中科院分区：
文献类型：
--
作者：
Tada, Y;Utsumi, S;Takaiwa, F
将在其胚乳中表达大豆甘氨酸的转基因水稻与两种类型的低谷蛋白突变体杂交，以确定蛋白质突变体的储存量可以有助于增加甘氨酸积累。在谷蛋白缺陷（即低谷蛋白）的遗传背景内，亲本转基因品系中的大豆球蛋白水平（102微克/100毫克种子）增强至大约224-237微克/100毫克种子。这种外源基因产物的富集通过其他内源谷醇溶蛋白和球蛋白储存蛋白表达的减少得到补偿，导致种子储存蛋白总量几乎相等。这些结果表明，低贮藏蛋白突变体可以为外源基因的表达提供潜在有用的宿主，从而允许更高水平的积累，因为它们可以为外源基因产物的积累提供比正常宿主植物更广泛的空间。
Transgenic rice expressing soybean glycinin in its endosperm was crossed with two types of low-glutelin mutants to determine how much storage the protein mutants can contribute to increases in glycinin accumulation. The glycinin level (102 mug/100 mg seed) in the parental transgenic line was enhanced to approximate to 224-237 mug/100 mg seed within a genetic background deficient in glutelin (i.e. of low glutelins). The enrichment of this foreign gene product was compensated by a decrease in the expression of other endogenous prolamine and globulin storage proteins, resulting in an almost equivalent total amount of seed storage proteins. These results show that low storage protein mutants can provide potentially useful hosts for the expression of foreign genes, allowing a higher-level accumulation, because they can provide wider space for the accumulation of foreign gene products than in the normal host plant.