REMOVALS OF HYDROGEN-PEROXIDE AND HYDROXYL RADICAL BY THIOL-SPECIFIC ANTIOXIDANT PROTEIN AS A POSSIBLE ROLE INVIVO
REMOVALS OF HYDROGEN-PEROXIDE AND HYDROXYL RADICAL BY THIOL-SPECIFIC ANTIOXIDANT PROTEIN AS A POSSIBLE ROLE INVIVO
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DOI：
10.1006/bbrc.1993.1410
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发表时间：
1993-04-15
影响因子：
3.1
通讯作者：
KIM, IH
中科院分区：
文献类型：
--
作者：
LIM, YS;CHA, MK;KIM, IH
研究发现，来自酿酒酵母的硫醇特异性抗氧化蛋白（保护蛋白；PRP）可以在二硫苏糖醇（DTT）的存在下去除过氧化氢和羟自由基。没有DTT作为还原当量，抗氧化蛋白没有表现出破坏过氧化氢和羟基自由基的活性。据观察，N-乙基马来酰亚胺 (NEM) 可以阻止 PRP 去除过氧化氢并保护 DNA 的裂解。这些观察结果表明PRP中半胱氨酸的巯基可以作为强亲核试剂来攻击和破坏H2O2和·OH。
Thiol-specific antioxidant protein (Protector Protein; PRP) fromSaccharomyces cerevisiaewas found to remove hydrogen peroxide and hydroxyl radical in the presense of dithiothreitol (DTT). Without DTT as a reducing equivalent, the antioxidant protein did not show the activities for destroying hydrogen peroxide and hydroxyl radical. N-ethylmaleimide (NEM) was observed to prevent the PRP from both removing hydrogen peroxide and protecting the cleavage of DNA. These observations suggest that the sulfhydryl of cysteine in PRP could function as a strong nucleophile to attack and destroy H2O2and ·OH.