Physiological strains induce differentiation in human osteoblasts cultured on orthopaedic biomaterial
Physiological strains induce differentiation in human osteoblasts cultured on orthopaedic biomaterial
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DOI：
10.1016/s0142-9612(03)00152-2
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发表时间：
2003-08-01
期刊：
影响因子：
14
通讯作者：
Rattner, A
中科院分区：
文献类型：
--
作者：
Di Palma, F;Douet, M;Rattner, A
我们建立了成骨细胞在金属生物材料上的体外机械拉伸模型，以研究机械应变对骨科种植体骨结合的影响。采用氧化铝或羟基磷灰石涂层的钛合金圆盘作为衬底。三个Dyacell(R)装置是专门设计用来对刚性生物材料施加循环应变的。根据生理数据和对髋部假体变形的估计，确定了治疗方案(600个Muepsilon应变，0.25 Hz)。这些装置的性能是可重复性的，并提供可控的变形。以人骨肉瘤细胞系MG-63、原代培养的人成骨细胞和ROS17/2.8大鼠骨肉瘤细胞为细胞模型。细胞生长和碱性磷酸酶(ALP)活性通过两种方案的原位检测来评估：每天重复三次15分钟的变形以模拟康复运动和24小时连续变形。结果表明，连续形变对MG-63细胞的生长和ALP活性没有影响，而连续形变对细胞数量没有影响，但在拉伸5天后刺激了ALP活性。这种序贯方案还可以改变人骨源性细胞的行为，导致5天后促进增殖，15天后刺激碱性磷酸酶(ALP)活性。连续变形的ROS17/2.8大鼠骨肉瘤细胞只有在拉伸1d后才能提高其ALP活性，从而比其他细胞株反应更快。与MG-63细胞一样，ROS17/2.8大鼠骨肉瘤细胞系的增殖不受连续变形的影响。这项研究表明，短的、重复的变形被定义为模仿假体植入后推荐的康复练习，比连续的应变更有可能对种植的骨产生有益的影响。(C)2003爱思唯尔科学有限公司。保留所有权利。
We have developed an in vitro mechanical stretching model of osteoblastic cells cultured on metallic biomaterials in order to study the effects of mechanical strain on osteointegration of orthopaedic implants. Titanium alloy discs coated with alumina or hydroxyapatite were used as substrates. Three Dynacell(R) devices were especially designed to apply cyclic strains on rigid biomaterials. The regimen (600 muepsilon strains, 0.25 Hz) was defined on the basis of physiological data and estimated deformation on hip stern prostheses. The performances of these apparatus were reproducible and provided controlled deformations. Human osteosarcoma cell line MG-63, human osteoblasts obtained from primary Cultures and ROS 17/2.8 rat osteosarcoma cells were used as cell models. Cell behaviour was assessed in terms of growth and alkaline phosphatase (ALP) activity by in situ assays for two regimens: 15-min deformations repeated three times a day to mimic rehabilitation exercises and 24-h continuous deformations. We demonstrated that continuous deformation did not affect the growth and ALP activity of MG-63 cells, in contrast with sequential deformations which had no effect on cell number, but which stimulated ALP activity after 5 days of stretching. This sequential regimen can also modify the behaviour of human bone-derived cells resulting in increased proliferation after 5 days and stimulation of ALP activity after 15 days. ROS 17/2.8 rat osteosarcoma cells submitted to sequential deformations responded faster than other cell lines by increasing their ALP activity only after I day of stretching. Like MG-63 cells, proliferation of the ROS 17/2.8 rat osteosarcoma cell line was not affected by sequential deformations. This study suggests that short, repeated deformations defined to mimic rehabilitation exercises recommended after prostheses implantation are more likely to exert beneficial effects on implanted bone than continuous strains. (C) 2003 Elsevier Science Ltd. All rights reserved.