First evidence of the activation of Cg-timp, an immune response component of pacific oysters, through a damage-associated molecular pattern pathway
First evidence of the activation of Cg-timp, an immune response component of pacific oysters, through a damage-associated molecular pattern pathway
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DOI：
10.1016/j.dci.2006.04.002
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发表时间：
2007-01-01
影响因子：
2.9
通讯作者：
Escoubas, J. M.
中科院分区：
文献类型：
--
作者：
Montagnani, C.;Avarre, J. C.;Escoubas, J. M.
在之前的工作中，我们鉴定了长蛸cPNA （Cg-timp）编码一种具有脊椎动物组织金属蛋白酶抑制剂（TIMP）所有特征的蛋白质。该基因的表达模式使我们提出Cg-timp是牡蛎伤口加热和防御机制的重要因素。在这里，我们描述了在活细菌或死细菌以及细菌分泌/排泄产物和金属蛋白酶的挑战下牡蛎中Cg-timp表达的分析。令人惊讶的是，细菌分泌/排泄产物激活Cg-timp基因表达，而热失活的产物则不会。为了解决Cg-timp基因激活的信号转导途径问题，我们分离并测序了Cg-timp启动子和上游区域。位于基因5'端两侧的1kb基因组DNA片段包含几个调控元件，特别是三个NF-kappa B结合位点。讨论了这些基序在Cg-timp基因调控中的潜在作用。(c) 2006 Elsevier Ltd.版权所有。
In a previous work, we characterized a Crassostrea gigas cPNA (Cg-timp) encoding a protein which presents all the features of vertebrate tissue inhibitor of metalloproteinase (TIMP). The expression pattern of this gene led us to propose that Cg-timp is an important factor in oyster wound heating and defense mechanisms. Here we describe the analysis of Cg-timp expression in oysters challenged by live or dead bacteria as well as by bacterial secretory/excretory products and metalloproteinase. Surprisingly, bacterial secretory/excretory products activate Cg-timp gene expression whereas heat-inactivated ones do not. To address the question of the signal transduction pathway involved in Cg-timp gene activation, we isolated and sequenced Cg-timp promoter and upstream region. A 1-kb genomic DNA fragment flanking the 5'-end of the gene contains several regulatory elements and notably three NF-kappa B binding sites. The potential involvement of these motifs in Cg-timp gene regulation is discussed. (c) 2006 Elsevier Ltd. All rights reserved.