Preparation of DNA-immobilized magnetic particles and their utilization as an accumulative material of metal ions
Preparation of DNA-immobilized magnetic particles and their utilization as an accumulative material of metal ions
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DOI：
10.1557/jmr.2016.14
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发表时间：
2016-02
影响因子：
2.7
通讯作者：
M. Yamada;Akira Fujisawa;K. Morishige;E. Hosono
中科院分区：
文献类型：
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作者：
M. Yamada;Akira Fujisawa;K. Morishige;E. Hosono
将DNA、磁性Fe_3O_4粒子和硅烷偶联剂双[3-（三甲氧基硅基）丙基]胺混合，制备了DNA固定化Fe_3O_4粒子（DNA-Fe-particles）。将DNA-无机杂化材料均匀地固定在直径约为450 nm的磁性Fe_3O_4粒子上。这些DNA-Fe-粒子在水中是稳定的。此外，我们可以简单地通过磁铁从水溶液中收集DNA-Fe颗粒。因此，我们证明了各种金属离子，如重金属和稀土金属离子，通过DNA-Fe-粒子的积累。结果，尽管这些DNA-Fe颗粒可以选择性地积累重金属和稀土金属离子，但这些材料不能积累轻金属离子，例如Mg（II）和Ca（II）离子。此外，金属离子积累的DNA-Fe-颗粒可以通过用乙二胺四乙酸水溶液洗涤它们而再循环。
DNA-immobilized Fe_3O_4 particles (DNA–Fe-particles) were prepared by mixing DNA, magnetic Fe_3O_4 particles, and the silane coupling reagent, bis[3-(trimethoxysilyl)propyl]amine. The DNA-inorganic hybrid material was uniformly immobilized onto magnetic Fe_3O_4 particles with the diameters of approximately 450 nm. These DNA-Fe-particles were stable in water. Additionally, we could simply collect the DNA-Fe-particles by a magnet from an aqueous solution. Therefore, we demonstrated the accumulation of various metal ions, such as heavy and rare-earth metal ions, by the DNA-Fe-particles. As a result, although these DNA-Fe-particles could selectively accumulate heavy and rare-earth metal ions, these materials could not accumulate the light metal ions, such as Mg(II) and Ca(II) ions. Furthermore, the metal ion-accumulated DNA-Fe-particles could be recycled by washing them with an aqueous ethylenediaminetetraacetic acid solution.