Proteomic profiling of human bone marrow mesenchymal stem cells under shear stress
Proteomic profiling of human bone marrow mesenchymal stem cells under shear stress
复制标题
剪切应力下人骨髓间充质干细胞的蛋白质组学分析
DOI：
10.1007/s11010-010-0432-7
复制
发表时间：
2010-08-01
影响因子：
4.3
通讯作者：
Dou, Kefeng
中科院分区：
文献类型：
--
作者：
Yi, Wei;Sun, Yang;Dou, Kefeng
间充质干细胞(MSCs)是一种很有前途的血管组织工程种子细胞。骨髓间充质干细胞作为种子细胞，移植后必须承受血液切应力。已有研究表明，流体剪应力对MSCs的增殖和分化具有调节作用。然而，流体剪切力对MSCs的影响，包括调节的蛋白质类型，仍然不是很清楚。在这项研究中，我们将人间充质干细胞(HMSCs)暴露在3dyn/cm2剪应力下6h，并使用蛋白质组学分析将它们与对照组进行比较。13个特定蛋白质受到切应力的影响，其中10个蛋白质表达上调。特别是剪切力诱导Annexin A2和GAPDH的表达持续增加，这两种蛋白已被特异地证明影响hMSCs的功能。在这里，我们提出了第一个比较蛋白质组分析剪切力对hMSCs的影响。
Mesenchymal stem cells (MSCs) are promising seed cells for tissue engineering of blood vessels. As seed cells, MSCs must endure blood fluid shear stress after transplantation. It has been shown that fluid shear stress can regulate the proliferation and differentiation of MSCs. However, the effects of fluid shear stress on MSCs including the types of proteins modulated are still not well understood. In this study, we exposed human mesenchymal stem cells (HMSCs) to 3 dyn/cm2shear stress for 6 h and compared them to a control group using proteomic analysis. Thirteen specific proteins were affected by shear stress, 10 of which were up-regulated. Shear stress especially induced sustained increases in the expression of Annexin A2 and GAPDH, which have been specifically shown to affect HMSCs function. We present here the first comparative proteome analysis of effect of shear stress on HMSCs.