Application of experimental stressors in laboratory rodents.
Application of experimental stressors in laboratory rodents.
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DOI：
10.1002/0471142301.ns0804s34
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发表时间：
2006-02-01
影响因子：
--
通讯作者：
Koob, George F
中科院分区：
文献类型：
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作者：
Heinrichs, Stephen C;Koob, George F
本单元提出了八个独立的压力实验室啮齿动物协议。应激诱导是研究建立和维持应激状态的神经和神经内分泌机制的关键要素。前四个程序，制动，电击脚，游泳，噪音，涉及急性暴露于有害刺激。接下来的三个程序，社会隔离，居民/入侵者的侵略，和母亲的剥夺，诱导社会破坏退出一组住房条件，攻击内的一个占主导地位的男性，或隔离的一个preweanling小狗从它的母亲，不熟悉的领土，分别。最后一个程序，睡眠剥夺，涉及到被动地拒绝睡眠的机会。提供支持协议，以解决需要的环境驯化和平静的程序之前，任何压力相关的研究（包括，对于大鼠，处理的动物作为一个平静的措施），并详细说明了一个简单的方法，量化的反应，以一个给定的压力，通过直接测量的水平的应激激素促肾上腺皮质激素（ACTH）和皮质酮。
This unit presents eight separate stressor protocols for laboratory rodents. Stress induction is a critical element in the study of neural and neuroendocrine mechanisms involved in establishing and maintaining a state of stress. The first four procedures, immobilization, footshock, swimming, and noise, involve acute exposure to noxious stimuli. The next three procedures, social isolation, resident/intruder aggression, and maternal deprivation, induce social disruption by withdrawal from a group housing condition, attack within the unfamiliar territory of a dominant male, or segregation of a preweanling pup from its mother, respectively. The final procedure, sleep deprivation, involves passive denial of the opportunity to sleep. Support protocols are provided to address the need for environmental acclimation and calming procedures prior to any stress-related studies (including, for rats, handling of the animals as a calming measure) and to detail a simple method of quantifying the response to a given stressor by direct measurement of levels of the stress hormones adrenocorticotropic hormone (ACTH) and corticosterone.