Novel multifunctional theranostic liposome drug delivery system: construction, characterization, and multimodality MR, near-infrared fluorescent, and nuclear imaging.
Novel multifunctional theranostic liposome drug delivery system: construction, characterization, and multimodality MR, near-infrared fluorescent, and nuclear imaging.
复制标题
DOI：
10.1021/bc300175d
复制
发表时间：
2012-06-20
影响因子：
4.7
通讯作者：
Bao, Ande
中科院分区：
文献类型：
--
作者：
Li, Shihong;Goins, Beth;Zhang, Lujun;Bao, Ande
脂质体是有效的脂质纳米颗粒药物递送系统，其也可以用非侵入性多模态成像剂功能化，其中每种模态提供不同的信息并且在诊断、监测疾病治疗和评价脂质体药物药代动力学方面具有协同优势。我们设计并构建了一种多功能治疗诊断脂质体药物递送系统，该系统集成了脂质体药物递送的多模态磁共振（MR）、近红外（NIR）荧光和核成像以及治疗监测和预测。制备的脂质体由DSPC/胆固醇/Gd-DOTADSPE/DOTA-DSPE组成，摩尔比为39：35：25：1，具有硫酸铵/pH梯度。将经修饰的NIR荧光示踪剂IRDye-DSPE有效地后插入到预制脂质体中。阿霉素可以有效地后负载到多功能脂质体中。多功能阿霉素脂质体也可以稳定地用99 mTc或64 Cu放射性标记，分别用于单光子发射计算机断层扫描（SPECT）或正电子发射断层扫描（PET）成像。MR图像显示瘤内注射后脂质体在裸鼠头颈部鳞状细胞癌（SCCHN）肿瘤异种移植瘤中的高分辨率微瘤内分布。NIR荧光、SPECT和PET图像也清楚地显示了多功能脂质体的高肿瘤内滞留或分布。这种多功能载药脂质体系统有希望用于疾病治疗诊断学，允许对它们的体内行为进行非侵入性多模态NIR荧光、MR、SPECT和PET成像，并利用每种模态的固有优势。
Liposomes are effective lipid nanoparticle drug delivery systems, which can also be functionalized with non-invasive multimodality imaging agents with each modality providing distinct information and having synergistic advantages in diagnosis, monitoring of disease treatment, and evaluation of liposomal drug pharmacokinetics. We designed and constructed a multifunctional theranostic liposomal drug delivery system, which integrated multimodality magnetic resonance (MR), near-infrared (NIR) fluorescent and nuclear imaging of liposomal drug delivery, and therapy monitoring and prediction. The pre-manufactured liposomes were composed of DSPC/cholesterol/Gd-DOTADSPE/DOTA-DSPE with the molar ratio of 39:35:25:1 and having ammonium sulfate/pH gradient. A lipidized NIR fluorescent tracer, IRDye-DSPE, was effectively post-inserted into the pre-manufactured liposomes. Doxorubicin could be effectively post-loaded into the multifunctional liposomes. The multifunctional doxorubicin-liposomes could also be stably radiolabeled with 99mTc or 64Cu for single photon emission computed tomography (SPECT) or positron emission tomography (PET) imaging, respectively. MR images displayed the high resolution micro-intratumoral distribution of the liposomes in squamous cell carcinoma of head and neck (SCCHN) tumor xenografts in nude rats after intratumoral injection. NIR fluorescent, SPECT and PET images also clearly showed either the high intratumoral retention or distribution of the multifunctional liposomes. This multifunctional drug carrying liposome system is promising for disease theranostics allowing non-invasive multimodality NIR fluorescent, MR, SPECT and PET imaging of their in vivo behavior and capitalizing on the inherent advantages of each modality.
登录
查看更多内容
DOI：
10.1124/jpet.103.059535
发表时间：
2004-02-01
影响因子：
3.5
作者：
Bao, AD;Goins, B;Phillips, WT
通讯作者：
Phillips, WT
DOI：
10.1016/j.jvir.2010.02.027
发表时间：
2010-08-01
影响因子：
2.9
作者：
French, J. Tyler;Goins, Beth;Bao, Ande
通讯作者：
Bao, Ande
影响因子：
4.7
作者：
Andreozzi, Erica;Seo, Jai Woong;Louie, Angelique
通讯作者：
Louie, Angelique
DOI：
10.1016/j.ejpb.2008.09.017
发表时间：
2009-06-01
影响因子：
4.9
作者：
Hak, Sjoerd;Sanders, Honorius M. H. F.;Nicolay, Klaas
通讯作者：
Nicolay, Klaas
DOI：
10.1007/978-1-60761-447-0_32
发表时间：
2010-01-01
期刊：
LIPOSOMES: METHODS AND PROTOCOLS, VOL 2
影响因子：
--
作者：
Goins, Beth;Bao, Ande;Phillips, William T.
通讯作者：
Phillips, William T.