Single-channel currents in renal tubules.
Single-channel currents in renal tubules.
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肾小管中的单通道电流。
DOI：
10.1152/ajprenal.1984.247.2.f380
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发表时间：
1984
期刊：
影响因子：
--
通讯作者：
Helman,SI
中科院分区：
文献类型：
--
作者：
Koeppen,BM;Beyenbach,KW;Helman,SI
使用膜片钳技术研究离体的兔肾皮质集合管。主细胞天然顶膜的千兆欧姆密封是从灌注了林格溶液的组织中获得的。不需要对组织进行酶处理或其他预处理。尽管可以获得切除的斑块，但所研究的斑块主要是细胞上类型。在保持电压在 +/- 100 mV 之间时，观察到了十分之几皮安范围内的单一电流。由于表观反转电位处于等于或接近 0 的保持电压，因此需要消耗组织。尽管可以获得切除的斑块，但所研究的斑块主要是细胞上类型。在保持电压在 +/- 100 mV 之间时，观察到了十分之几皮安范围内的单一电流。由于表观反转电位处于等于或接近 0 的保持电压，因此需要消耗组织。尽管可以获得切除的斑块，但所研究的斑块主要是细胞上类型。在保持电压在 +/- 100 mV 之间时，观察到了十分之几皮安范围内的单一电流。由于表观反转电位处于或接近 0 mV 的保持电压，并且由于电流-电压关系明显非线性，因此单一电流很可能是由 K+ 引起的。如果存在 Na+ 通道电流波动，则在阿米洛利存在或不存在的情况下无法唯一识别。
Patch-clamp techniques were used to study isolated renal cortical collecting ducts of rabbits. Gigaohm seals of the native apical membranes of the principal cells were obtained from tissues superfused with a Ringer solution. No enzymatic or other pretreatment of the tissues was required. The patches studied were primarily of the on-cell type, although excised patches could be obtained. Unitary currents in a range of tenths of picoamperes were observed at holding voltages between +/- 100 mV. Since the apparent reversal potential was at a holding voltage at or near 0 eatment of the tissues was required. The patches studied were primarily of the on-cell type, although excised patches could be obtained. Unitary currents in a range of tenths of picoamperes were observed at holding voltages between +/- 100 mV. Since the apparent reversal potential was at a holding voltage at or near 0 eatment of the tissues was required. The patches studied were primarily of the on-cell type, although excised patches could be obtained. Unitary currents in a range of tenths of picoamperes were observed at holding voltages between +/- 100 mV. Since the apparent reversal potential was at a holding voltage at or near 0 mV and since the current-voltage relationship was markedly nonlinear, the unitary currents are most likely due to K+ . Na+-channel current fluctuations, if present, could not be uniquely identified in the presence or absence of amiloride.