Medicago LINC Complexes Function in Nuclear Morphology, Nuclear Movement, and Root Nodule Symbiosis.
Medicago LINC Complexes Function in Nuclear Morphology, Nuclear Movement, and Root Nodule Symbiosis.
复制标题
苜蓿 LINC 复合物在核形态、核运动和根瘤共生中发挥作用。
DOI：
10.1104/pp.18.01111
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发表时间：
2019
期刊：
影响因子：
7.4
通讯作者：
Newman-Griffis AH
中科院分区：
文献类型：
--
作者：
Newman-Griffis AH
核运动参与了真核生物生命中的细胞和发育过程，通常由核骨架和细胞骨架(LINC)复合体的连接物驱动，LINC复合体通过Klarsicht/ANC-1/Syne-1同源(KASH)和SAD1/UNC-84(SUN)蛋白的相互作用连接核膜(NE)。拟南芥LINC复合体参与多种细胞的核运动和定位。自20世纪50年代以来的观察描述了豆科植物和根瘤菌之间在共生启动过程中的有针对性的核运动和定位，但这些运动是功能性的还是偶然的还没有确定。在这里，我们识别和表征了模式豆科牧草中的LINC复合体。我们发现LINC复合体的特征，如NE的定位，Kash蛋白对太阳蛋白结合NE的富集性，以及SUN-Kash的直接结合，在植物之间是保守的。使用太阳显性-负策略，我们证明了LINC复合体是Medicagoroot毛发正确的核形状和运动所必需的，并且对于侵染线的启动和结瘤是重要的。
Nuclear movement is involved in cellular and developmental processes across eukaryotic life, often driven by Linker of Nucleoskeleton and Cytoskeleton (LINC) complexes, which bridge the nuclear envelope (NE) via the interaction of Klarsicht/ANC-1/Syne-1 Homology (KASH) and Sad1/UNC-84 (SUN) proteins. Arabidopsis (Arabidopsis thaliana) LINC complexes are involved in nuclear movement and positioning in several cell types. Observations since the 1950s have described targeted nuclear movement and positioning during symbiosis initiation between legumes and rhizobia, but it has not been established whether these movements are functional or incidental. Here, we identify and characterize LINC complexes in the model legumeMedicago truncatula. We show that LINC complex characteristics such as NE localization, dependence of KASH proteins on SUN protein binding for NE enrichment, and direct SUN-KASH binding are conserved between plant species. Using a SUN dominant-negative strategy, we demonstrate that LINC complexes are necessary for proper nuclear shaping and movement inMedicagoroot hairs, and are important for infection thread initiation and nodulation.