Spectral reflectance indices and pigment functions during leaf ontogenesis in six subtropical landscape plants
Spectral reflectance indices and pigment functions during leaf ontogenesis in six subtropical landscape plants
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DOI：
10.1007/s10725-008-9353-9
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发表时间：
2009-05
影响因子：
4.2
通讯作者：
Nan Liu;Zhi-fang Lin;Anna Van Devender;G. Lin;C. Peng;Xiaoyan Pan;Shao-Wei Chen;Qun Gu
中科院分区：
文献类型：
--
作者：
Nan Liu;Zhi-fang Lin;Anna Van Devender;G. Lin;C. Peng;Xiaoyan Pan;Shao-Wei Chen;Qun Gu
色素组合在叶的个体发育过程中受到调控。为了更好地了解色素的功能，研究了龙船花、山茶花、丁香、芒果花等6种亚热带园林植物叶片发育过程中叶绿素、类胡萝卜素和花青素含量的变化，桂花香和Saraca潜水皮埃尔。高浓度的花青素苷与幼叶叶绿素的减少有关。随着叶片的发育，所有六种植物的光合色素（叶绿素和类胡萝卜素）增加，而花青素浓度下降。叶绿素荧光成像（ΦPSII）和非光化学荧光猝灭（NPQ）以及电子传递速率-快速光变曲线（RLC）的测定结果表明，叶片电子传递速率（ETR）、Φ PSII和RLC饱和点随叶片发育而增加，但随年龄的增长而下降。幼叶的NPQ和Car/Chl比值高于其他发育阶段的叶片。测量叶片反射光谱（400-800 nm）以提供个体发育期间叶片中色素的体内非破坏性评估。四个反射率指数，相关的色素性状进行了比较，从生化分析定量获得的数据。结果表明，花青苷反射指数（ARI）与幼叶花青苷浓度呈线性相关，叶绿素归一化差异植被指数（Chl NDI）与叶绿素浓度呈显著正相关。光合反射指数与Car/Chl比值关系不密切，而与结构无关的色素指数则不受叶片发育或物种的影响。因此，幼叶中高浓度的花色素苷、较高的NPQ和Car/Chl比值是光合机构未发育完全时科普高辐射的重要功能反应。另外两个叶片反射率指数ARI和Chl NDI可用于叶片发育过程中的活体色素评价。
Pigment combinations are regulated during leaf ontogenesis. To better understand pigment function, alterations in chlorophyll, carotenoid and anthocyanin concentrations were investigated during different leaf development stages in six subtropical landscape plants, namelyIxora chinensisLam,Camellia japonicaLinn,Eugenia oleinaWight,Mangifera indicaL.,Osmanthus fragransLowr andSaraca divesPierre. High concentrations of anthocyanin were associated with reduced chlorophyll in juvenile leaves. As leaves developed, the photosynthetic pigments (chlorophyll and carotenoid) of all six species increased while anthocyanin concentration declined. Chlorophyll fluorescence imaging of ΦPSII(effective quantum yield of PSII) and of NPQ (non-photochemical fluorescence quenching) and determination of electron transport rate-rapid light curve (RLC) showed that maximum ETR (leaf electron transport rate), ΦPSIIand the saturation point in RLC increased during leaf development but declined as they aged. Juvenile leaves displayed higher values of NPQ and Car/Chl ratios than leaves at other developmental stages. Leaf reflectance spectra (400–800 nm) were measured to provide an in vivo non-destructive assessment of pigments in leaves during ontogenesis. Four reflectance indices, related to pigment characters, were compared with data obtained quantitatively from biochemical analysis. The results showed that the ARI (anthocyanin reflectance index) was linearly correlated to anthocyanin concentration in juvenile leaves, while a positive correlation of Chl NDI (chlorophyll normalized difference vegetation index) to chlorophyllaconcentration was species dependent. Photosynthetic reflectance index was not closely related to Car/Chl ratio, while a structural-independent pigment index was not greatly altered by leaf development or species. Accordingly, it is suggested that the high concentration of anthocyanin, higher NPQ and Car/Chl ratio in juvenile leaves are important functional responses to cope with high radiation when the photosynthetic apparatus is not fully developed. Another two leaf reflectance indices, ARI and Chl NDI, are valuable for in vivo pigment evaluation during leaf development.