MY01, a class XIV myosin, affects developmentally‐regulated elimination of the macronucleus during conjugation of Tetrahymena thermophila
MY01, a class XIV myosin, affects developmentally‐regulated elimination of the macronucleus during conjugation of Tetrahymena thermophila
复制标题
MY01 是一种 XIV 类肌球蛋白，影响嗜热四膜虫接合过程中大核的发育调节消除
DOI：
10.1042/bc20080198
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发表时间：
2009
影响因子：
2.7
通讯作者：
Ray H. Gavin
中科院分区：
文献类型：
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作者：
J. Garcés;R. E. Hosein;Ray H. Gavin
背景资料。核二型是纤毛原生动物的特征。转录活性大核与转录沉默微核共存，后者仅在接合时被激活。在接合过程中，每个接合子产生两个新的遗传匹配的大核和微核，并且消除先前存在的大核。结合过程中预先存在的大核的消除是一种凋亡样过程。大核变得高度浓缩，DNA梯状化发生，半胱天冬酶活性增加，酸性酶在核质内积累，并且核的大小缩小。目前的研究集中在肌动蛋白和肌球蛋白的参与核事件的共轭。将肌球蛋白敲除菌株与野生型交配，并用共聚焦显微镜监测核事件。
Background information. Nuclear dimorphism is characteristic of ciliated protozoa. A transcriptionally‐active macronucleus co‐exists with a transcriptionally‐silent micronucleus, which is activated only at conjugation. During conjugation, each conjugant develops two new genetically matched macronuclei and micronuclei, and the pre‐existing macronucleus is eliminated. Elimination of the pre‐existing macronucleus during conjugation is an apoptotic‐like process. The macronucleus becomes highly condensed, DNA laddering occurs, caspase activity increases, acidic enzymes accumulate within the nucleoplasm, and the nucleus shrinks in size. The current study focused on the involvement of actin and myosin in nuclear events of conjugation. A myosin knockout strain was mated with wild‐type, and the nuclear events were monitored with confocal microscopy.
影响因子：
--
作者：
Eduardo Orias;E. Hamilton;Judith D. Orias
通讯作者：
Eduardo Orias;E. Hamilton;Judith D. Orias
影响因子：
2.7
作者：
DAVIS, MC;WARD, JG;ALLIS, CD
通讯作者：
ALLIS, CD