Subcellular localization of benzodiazepine/GABAA receptors in the cerebellum of rat, cat, and monkey using monoclonal antibodies
Subcellular localization of benzodiazepine/GABAA receptors in the cerebellum of rat, cat, and monkey using monoclonal antibodies
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使用单克隆抗体对大鼠、猫和猴子小脑中苯二氮卓/GABAA 受体进行亚细胞定位
DOI：
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发表时间：
1989
影响因子：
5.3
通讯作者：
Mr Frank Kennedy
中科院分区：
文献类型：
--
作者：
Peter Somogyi;Hiroshi Takagi;J. Grayson Richards;H. Mohler;Miss Patty Mcchlery;Mr David Roberts;Mr Frank Kennedy
两种单克隆抗体，bd-17和bd-24，特异性的β-和α-亚基的GABAA/苯二氮卓受体/氯离子通道复合物，分别，被用来确定免疫反应性的亚细胞分布在小脑的电子显微镜。2种抗体在质膜上显示出相似的抗原分布（大鼠除外; bd-24不识别大鼠抗原），但α亚基的细胞内免疫反应性更普遍。所有神经元类型的质膜均呈免疫阳性。不同类型的细胞之间的免疫反应性的程度差异很大，但它是定型的同一类型的单个细胞之间。颗粒细胞表现出最强的免疫反应性，不仅在其接受来自含GABA的高尔基细胞末端的突触的树突上，而且在其不接受突触的胞体上。星状细胞和篮状细胞的免疫反应性较弱。浦肯野细胞在其体细胞膜上呈弱阳性反应，但在树突轴和树突棘上呈强阳性反应。高尔基体细胞的免疫反应性可忽略不计。小脑深核的神经元沿其质膜呈强免疫阳性沿着。免疫反应性很强，在内质网池和高尔基囊的星状细胞和篮状细胞，变量浦肯野细胞，而颗粒细胞很少免疫反应性胞内。有人建议，这些差异反映了不同类型的细胞中的受体复合物的营业额的差异。由GABA能星状细胞、篮状细胞和高尔基体细胞的扣结建立的突触裂隙呈免疫阳性，小脑深核中的许多突触也呈免疫阳性。然而，免疫反应性也存在沿着非交界质膜，并得出结论，这反映了抗原的分布。在假定的谷氨酸释放平行和苔藓纤维终端的突触裂缝几乎总是免疫阴性。在轴突、神经末梢或神经胶质细胞上未检测到免疫反应性。结果表明，不同类型的神经元表达GABAA/苯二氮卓受体/氯离子通道复合物的程度不同。受体复合物的分布表明，GABA能影响的细胞拓扑结构不是由受体的精确空间排列决定的，而是由GABA释放终末的精确位置决定的，这是小脑回路的一个特征。
Two monoclonal antibodies, bd-17 and bd-24, specific for the beta- and alpha-subunit of the GABAA/benzodiazepine receptor/chloride channel complex, respectively, were used to determine the subcellular distribution of immunoreactivity in the cerebellum by electron microscopy. The 2 antibodies showed similar antigen distribution on the plasma membrane (except in the rat; bd-24 does not recognize the rat antigen), but intracellular immunoreactivity was more prevalent for the alpha-subunit. The plasma membrane of all neuronal types was immunopositive. The degree of immunoreactivity varied greatly between different types of cell, but it was stereotyped among individual cells of the same type. Granule cells showed the strongest immunoreactivity, not only on their dendrites which receive synapses from GABA-containing Golgi cell terminals, but also on their somata which do not receive synapses. Stellate and basket cells were somewhat weaker in immunoreactivity. Purkinje cells were only weakly positive on their somatic membrane but stronger on their dendritic shafts and spines. Golgi cells showed negligible if any immunoreactivity. Neurons of the deep cerebellar nuclei were strongly immunopositive along their plasma membrane. Immunoreactivity was strong in cisternae of the endoplasmic reticulum and in the Golgi saccules of stellate and basket cells, variable in Purkinje cells, while granule cells were rarely immunoreactive intracellularly. It is suggested that these differences reflect differences in the turnover of the receptor complex in the different cell types. The synaptic clefts established by boutons of the GABAergic stellate, basket, and Golgi cells were immunopositive, as were many synapses in the deep cerebellar nuclei. However, immunoreactivity was also present along the nonjunctional plasma membrane, and it was concluded that this reflected the distribution of the antigen. The synaptic clefts at the presumed glutamate-releasing parallel and mossy fiber terminals were almost always immunonegative. No immunoreactivity was detected on axons, nerve terminals, or glial cells. The results demonstrate that different neuronal types express the GABAA/benzodiazepine receptor/chloride channel complex to different degrees. The distribution of the receptor complex suggests that the cellular topography of GABAergic influence is not governed by the precise spatial arrangement of the receptors but by the precise placement of the GABA-releasing terminals, a characteristic of the cerebellar circuit.
苯二氮卓受体与 γ-氨基丁酸 (GABA) 受体亚群偶联：来自定量放射自显影研究的证据。
DOI：
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发表时间：
1981
期刊：
The Journal of pharmacology and experimental therapeutics
影响因子：
--
作者：
Unnerstall,JR;Kuhar,MJ;Niehoff,DL;Palacios,JM
通讯作者：
Palacios,JM
影响因子：
3.6
作者：
R. Squires;J. Casida;M. Richardson;E. Saederup
通讯作者：
R. Squires;J. Casida;M. Richardson;E. Saederup
脊椎动物神经元原代培养物中苯二氮卓受体的分布、移动性和功能。
DOI：
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发表时间：
1988
期刊：
Advances in biochemical psychopharmacology
影响因子：
--
作者：
Thompson,CL;Angelides,KJ;Velazquez,JL;BarnesJr,EM
通讯作者：
BarnesJr,EM