Genetic elimination of dengue vector mosquitoes
Genetic elimination of dengue vector mosquitoes
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DOI：
10.1073/pnas.1019295108
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发表时间：
2011-03-22
影响因子：
11.1
通讯作者：
Black, William C.
中科院分区：
文献类型：
--
作者：
de Valdez, Megan R. Wise;Nimmo, Derric;Black, William C.
正在开发一种基于携带条件显性致死基因的蚊子（释放携带显性致死基因 RIDL 的昆虫）的方法，通过抑制媒介种群来控制登革热病毒的传播。主要登革热载体埃及伊蚊的转基因菌株被命名为 OX3604C，经过工程改造，具有可抑制的雌性特异性不飞表型。该菌株避免了辐射诱导绝育的需要，允许基因性别鉴定，导致仅释放雄性，并允许释放卵而不是成年蚊子。每周将 OX3604C 雄性蚊子引入含有稳定目标蚊子种群的大型实验室笼子中，初始比例为 8.5-10:1 OX3604C: 目标蚊子在 10-20 周内消除了种群。这些数据支持在封闭或封闭的田间试验中进一步测试该菌株，以评估交配竞争力以及环境和其他影响。成功完成现场试验应有助于将这种方法纳入大面积登革热控制或消除工作，作为综合病媒管理战略的组成部分。
An approach based on mosquitoes carrying a conditional dominant lethal gene (release of insects carrying a dominant lethal, RIDL) is being developed to control the transmission of dengue viruses by vector population suppression. A transgenic strain, designated OX3604C, of the major dengue vector, Aedes aegypti, was engineered to have a repressible female-specific flightless phenotype. This strain circumvents the need for radiation-induced sterilization, allows genetic sexing resulting in male-only releases, and permits the release of eggs instead of adult mosquitoes. OX3604C males introduced weekly into large laboratory cages containing stable target mosquito populations at initial ratios of 8.5-10:1 OX3604C:target eliminated the populations within 10-20 weeks. These data support the further testing of this strain in contained or confined field trials to evaluate mating competitiveness and environmental and other effects. Successful completion of the field trials should facilitate incorporation of this approach into area-wide dengue control or elimination efforts as a component of an integrated vector management strategy.