Eradication of established murine tumors using a novel cell-free vaccine: dendritic cell-derived exosomes
Eradication of established murine tumors using a novel cell-free vaccine: dendritic cell-derived exosomes
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DOI：
10.1038/nm0598-594
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发表时间：
1998-05-01
期刊：
影响因子：
82.9
通讯作者：
Amigorena, S
中科院分区：
文献类型：
--
作者：
Zitvogel, L;Regnault, A;Amigorena, S
树突状细胞（Dendritic cells，DC）是一种专职的抗原提呈细胞，具有独特的诱导机体初次和二次免疫应答的能力。在这里，我们表明，树突状细胞分泌抗原呈递囊泡，称为外来体，表达功能的主要组织相容性复合物I类和II类，和T细胞共刺激分子。肿瘤肽脉冲DC衍生的外来体在体内引发特异性细胞毒性T淋巴细胞，并以T细胞依赖性方式根除或抑制已建立的鼠肿瘤的生长。基于外来体的无细胞疫苗代表了DC过继疗法的替代方案，用于抑制肿瘤生长。
Dendritic cells (DCs) are professional antigen presenting cells with the unique capacity to Induce primary and secondary immune responses in vivo. Here, we show that DCs secrete antigen presenting vesicles, called exosomes, which express functional Major Histocompatibility Complex class I and class II, and T-cell costimulatory molecules. Tumor peptide-pulsed DC-derived exosomes prime specific cytotoxic T lymphocytes in vivo and eradicate or suppress growth of established murine tumors in a T cell-dependent manner. Exosome-based cell-free vaccines represent an alternative to DC adoptive therapy for suppressing tumor growth.