Responsive Inverse Opal Hydrogels for the Sensing of Macromolecules
Responsive Inverse Opal Hydrogels for the Sensing of Macromolecules
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DOI：
10.1002/anie.201500674
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发表时间：
2015-05-26
影响因子：
16.6
通讯作者：
Wischerhoff, Erik
中科院分区：
文献类型：
--
作者：
Couturier, Jean-Philippe;Suetterlin, Martin;Wischerhoff, Erik
双响应反蛋白石水凝胶被设计为（生物）大分子的自主传感器系统，利用分析物诱导的蛋白石的结构颜色的调制。基于低聚（乙二醇）大分子单体的体系另外引入具有各种识别单元的共聚单体。它们联合收割机了LCST类型的线圈到小球的塌陷转变与转变温度对分子识别过程的敏感性。这使得能够通过简单的光学方法特异性检测大分子分析物，如糖聚合物和蛋白质。虽然反蛋白石结构有助于大的分析物有效扩散到光子晶体中，但在给定温度下分析物结合时，刺激响应性引起光学布拉格峰超过100 nm的强烈位移。该系统的设计为开发易于使用、快速且低成本的病原体传感器提供了一个通用平台。
Dual responsive inverse opal hydrogels were designed as autonomous sensor systems for (bio)macromolecules, exploiting the analyte-induced modulation of the opal's structural color. The systems that are based on oligo(ethylene glycol) macromonomers additionally incorporate comonomers with various recognition units. They combine a coil-to-globule collapse transition of the LCST type with sensitivity of the transition temperature toward molecular recognition processes. This enables the specific detection of macromolecular analytes, such as glycopolymers and proteins, by simple optical methods. While the inverse opal structure assists the effective diffusion even of large analytes into the photonic crystal, the stimulus responsiveness gives rise to strong shifts of the optical Bragg peak of more than 100nm upon analyte binding at a given temperature. The systems' design provides a versatile platform for the development of easy-to-use, fast, and low-cost sensors for pathogens.