Rad52 protein has a second stimulatory role in DNA strand exchange that complements replication protein-A function
Rad52 protein has a second stimulatory role in DNA strand exchange that complements replication protein-A function
复制标题
DOI：
10.1074/jbc.m203670200
复制
发表时间：
2002-07-19
影响因子：
4.8
通讯作者：
Kowalczykowski, SC
中科院分区：
文献类型：
--
作者：
New, JH;Kowalczykowski, SC
Rad52蛋白在酿酒酵母双链断裂修复和同源重组中起着重要作用。我们已经确定了一种新的机制，Rad52蛋白刺激Rad51蛋白促进的DNA链交换。当使用亚饱和量（相对于单链DNA浓度）的复制蛋白A（RPA）时，Rad52蛋白的这种功能被揭示。在这些条件下，需要Rad52蛋白进行广泛的DNA链交换。有趣的是，在这个新的作用，Rad52蛋白既不作为一个简单的单链DNA结合蛋白本身，也不，在其以前确定的刺激作用，它需要与RPA的物理相互作用，因为它可以取代大肠杆菌单链DNA结合蛋白。我们认为Rad52蛋白通过稳定Rad51突触前丝发挥作用。
Rad52 protein plays a central role in double strand break repair and homologous recombination in Saccharomyces cerevisiae. We have identified a new mechanism by which Rad52 protein stimulates Rad51 protein-promoted DNA strand exchange. This function of Rad52 protein is revealed when subsaturating amounts (relative to the single-stranded DNA concentration) of replication protein-A (RPA) are used. Under these conditions, Rad52 protein is needed for extensive DNA strand exchange. Interestingly, in this new role, Rad52 protein neither acts simply as a single strand DNA-binding protein per se nor, in contrast to its previously identified stimulatory roles, does it require physical interaction with RPA because it can be substituted by the Escherichia coli single strand DNA-binding protein. We propose that Rad52 protein acts by stabilizing the Rad51 presynaptic filament.