Real-time in-cell 19F NMR study on uptake of fluorescent and nonfluorescent 19F-octaarginines into human Jurkat cells
Real-time in-cell 19F NMR study on uptake of fluorescent and nonfluorescent 19F-octaarginines into human Jurkat cells
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DOI：
10.1246/cl.2005.1064
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发表时间：
2005-07-05
影响因子：
1.6
通讯作者：
Nakahara, M
中科院分区：
文献类型：
--
作者：
Okamura, E;Ninomiya, K;Nakahara, M
实时细胞内(FNMR)-F-19光谱用于确定细胞摄取荧光和非荧光f -19-辛精氨酸的时间尺度。在1-2min的时间分辨率下，成功检测到低至10 μ M量级的肽。浓度分析表明，异硫氰酸荧光素（FITC）诱导肽进入天然人体细胞的不同动力学。（FNMR）-F-19检测生物活性分子实时摄取的能力显示出最小的扰动。
Real-time in-cell (FNMR)-F-19 spectroscopy is developed to determine the time scale of cellular uptake of fluorescent and nonfluorescent F-19-octaarginines. The peptide concentration as low as 10-mu M order is successfully detected with a time resolution of 1-2min. The concentration analysis shows that fluorescein isothiocyanate (FITC) induces different kinetics for the peptide uptake into natural human cells. The ability of (FNMR)-F-19 to detect the real-time uptake of bioactive molecules is shown with minimal perturbation.