A TRANS-ACTING SUPPRESSOR RESTORES SPLICING OF A YEAST INTRON WITH A BRANCH POINT MUTATION
A TRANS-ACTING SUPPRESSOR RESTORES SPLICING OF A YEAST INTRON WITH A BRANCH POINT MUTATION
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DOI：
10.1101/gad.1.5.445
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发表时间：
1987-07-01
影响因子：
10.5
通讯作者：
GUTHRIE, C
中科院分区：
文献类型：
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作者：
COUTO, JR;TAMM, J;GUTHRIE, C
来自酿酒酵母前mRNA的内含子的剪接需要保守序列TACTAAC;最3 '端的A残基被用作分支形成的位点。我们以前表明，转录自肌动蛋白-HIS 4基因融合含有突变TACTAAC到TACTACC（指定C259）的剪接效率低下，从而防止组氨酸前体组氨酸上的生长。通过选择生长组氨醇，我们已经确定了一个突变体，其中C259转录的剪接增加了四倍，其他突变内含子的剪接没有显着改善。突变基因座编码反式作用抑制基因。一个单一的突变，rna 16 -1，是足够的抑制，然而，抑制是最大化的杂合二倍体含有rna 16 -1和野生型等位基因RNA 16。此外，野生型前体mRNA（和lymphocyte中间体）在rna 16 -1细胞中积累。我们建议RNA 16位点编码剪接机制的一个组成部分。
Splicing of introns from Saccharomyces cerevisiae pre-mRNA requires the conserved sequence TACTAAC; the 3'-most A residue is utilized as the site of branch formation. We showed previously that the transcript from an actin-HIS4 gene fusion containing the mutation TACTAAC to TACTACC (designated C259) is spliced inefficiently, thereby preventing growth on the histidine precursor histidinol. By selecting for growth on histidinol, we have identified a mutant in which the splicing of the C259 transcript is increased fourfold; splicing of other mutated introns is not significantly improved. The mutant locus encodes a trans-acting suppressor. A single mutation, rna16-1, is sufficient for suppression; however, suppression is maximized in heterozygous diploids containing both rna16-1 and the wild-type allele RNA16. In addition, wild-type pre-mRNAs (and lariat intermediates) accumulate in rna16-1 cells. We propose that the RNA16 locus encodes a component of the splicing machinery.