Lex glycan mediates homotypic adhesion of embryonal cells independently from E-cadherin:: A preliminary note
Lex glycan mediates homotypic adhesion of embryonal cells independently from E-cadherin:: A preliminary note
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DOI：
10.1016/j.bbrc.2007.04.101
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发表时间：
2007-06-22
影响因子：
3.1
通讯作者：
Hakomori, Sen-itiroh
中科院分区：
文献类型：
--
作者：
Handa, Kazuko;Takatani-Nakase, Tomoka;Hakomori, Sen-itiroh
Le(x)聚糖和e -钙粘蛋白（Ecad）在胚胎干细胞（ES）和胚胎癌（EC）细胞中共表达。虽然Ecad介导这些细胞的同型粘附的结构和功能已经很好地建立起来，但证明Le(x)聚糖也介导这种粘附的证据很弱，尽管Le(x)寡糖抑制了压实过程。为了提供更有力的证据，我们敲除EC和ES细胞中的Ecad基因，建立了F9 Ecad（-/-）和D3M Ecad（-/-）细胞，它们高度表达Le(x)聚糖，但根本不表达Ecad。F9 Ecad（-/-）和D3M Ecad（-/-）细胞在Ca2+存在下均表现出较强的自聚集，而PYS-2细胞表达微量Ecad和无法检测到的Lex聚糖，不表现出自聚集。此外，在Ca2+存在的情况下，F9 Ecad（-/-）和D3M Ecad（-/-）细胞对涂有Lex鞘糖脂（III3 FucnLc4Cer）的板表现出剂量依赖性的强粘附。因此，ES或EC细胞在没有Ecad的情况下表现出自聚集和对Le(x)涂层板的强粘附，进一步支持了细胞粘附中Lex自我识别(即Le（x)与Le(x)相互作用）的概念。(c) 2007爱思唯尔公司版权所有。
Le(x) glycan and E-cadherin (Ecad) are co-expressed at embryonal stem (ES) cells and embryonal carcinoma (EC) cells. While the structure and function of Ecad mediating homotypic adhesion of these cells have been well established, evidence that Le(x) glycan also mediates such adhesion is weak, despite the fact that Le(x) oligosaccharide inhibits the compaction process. To provide stronger evidence, we knocked out Ecad gene in EC and ES cells to establish F9 Ecad (-/-) and D3M Ecad (-/-) cells, which highly express Le(x) glycan but do not express Ecad at all. Both F9 Ecad (-/-) and D3M Ecad (-/-) cells displayed strong autoaggregation in the presence of Ca2+, while PYS-2 cells, which express trace amount of Ecad and undetectable level of Lex glycan, did not display autoaggregation. In addition, F9 Ecad (-/-) and D3M Ecad (-/-) cells displayed strong adhesion to plates coated with Lex glycosphingolipid (III3 FucnLc4Cer), in dose-dependent manner, in the presence of Ca2+. Thus, ES or EC cells display autoaggregation and strong adhesion to Le(x)-coated plates in the absence of Ecad, further supporting the notion of Lex self-recognition (i.e., Le(x)-to-Le(x) interaction) in cell adhesion. (c) 2007 Elsevier Inc. All rights reserved.