Selective partitioning of dietary fatty acids into the VLDL TG pool in the early postprandial period
Selective partitioning of dietary fatty acids into the VLDL TG pool in the early postprandial period
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DOI：
10.1194/jlr.m300167-jlr200
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发表时间：
2003-11-01
影响因子：
6.5
通讯作者：
Frayn, KN
中科院分区：
文献类型：
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作者：
Heath, RB;Karpe, F;Frayn, KN
循环三酰甘油（TG）主要来自膳食脂肪。然而，人们对膳食脂肪进入主要的TG库，即极低密度脂蛋白（VLDL）TG知之甚少。我们使用了一种新的方法来研究膳食脂肪酸的具体纳入餐后极低密度脂蛋白TG在人类。8名健康志愿者（年龄25.4 ± 2.2岁，体重指数22.1 ± 2.3 kg/m2）被喂食含有30 g鱼油和600 mg [1-C-13]棕榈酸的混合餐。使用抗载脂蛋白B-100的免疫亲和性分离乳糜微粒和VLDL。脂蛋白的脂肪酸组成通过气相色谱/质谱分析。[1-C-13]棕榈酸在进食后3小时开始出现在VLDL TG中，二十碳五烯酸（EPA）和二十二碳六烯酸（DHA）也观察到类似的延迟。大约20%的膳食脂肪酸进入VLDL TG池6小时后，膳食摄入。与[1-C-13]棕榈酸和EPA相比，DIFIA明显过量掺入该池中。这似乎取决于这种脂肪酸在非酯化脂肪酸池中的显著升高。jlr总之，膳食脂肪酸对早期餐后极低密度脂蛋白TG的贡献是巨大的。DIFIA在VLDL TG生产中的作用需要进一步研究。希思河B.，F.卡佩河W. Milne，G. C. Burdge，S. A. Wootton和K. N.弗莱恩在餐后早期，膳食脂肪酸选择性地分配到VLDL TG库中。
Circulating triacylglycerol (TG) arises mainly from dietary fat. However, little is known about the entry of dietary fat into the major TG pool, very low-density lipoprotein (VLDL) TG. We used a novel method to study the specific incorporation of dietary fatty acids into postprandial VLDL TG in humans. Eight healthy volunteers (age 25.4 +/- 2.2 years, body mass index 22.1 +/- 2.3 kg/m(2)) were fed a mixed meal containing 30 g fish oil and 600 mg [1-C-13]palmitic acid. Chylomicrons and VLDL were separated using immunoaffinity against apolipoprotein B-100. The fatty acid composition of lipoproteins was analyzed by gas chromatography/mass spectrometry. [1-C-13]palmitic acid started to appear in VLDL TG 3 h after meal intake, and a similar delay was observed for eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA). Approximately 20% of dietary fatty acids entered the VLDL TG pool 6 h after meal intake. DIFIA was clearly overincorporated into this pool compared with [1-C-13]palmitic acid and EPA. This seemed to depend on a marked elevation of this fatty acid in the nonesterified fatty acid pool.jlr In summary, the contribution of dietary fatty acids to early postprandial VLDL TG is substantial. The role of DIFIA in VLDL TG production will require further investigation.-Heath, R. B., F. Karpe, R. W. Milne, G. C. Burdge, S. A. Wootton, and K. N. Frayn. Selective partitioning of dietary fatty acids into the VLDL TG pool in the early postprandial period.