Genome-Wide Identification and Expression Analysis of WRKY Gene Family in Capsicum annuum L.
Genome-Wide Identification and Expression Analysis of WRKY Gene Family in Capsicum annuum L.
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DOI：
10.3389/fpls.2016.00211
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发表时间：
2016
影响因子：
5.6
通讯作者：
Wei G
中科院分区：
文献类型：
--
作者：
Diao WP;Snyder JC;Wang SB;Liu JB;Pan BG;Guo GJ;Wei G
WRKY转录因子家族是植物转录调控因子中最重要的家族之一，其成员调控多种生物学过程，尤其是对生物和非生物胁迫的防御。然而，很少有关于辣椒（辣椒）WRKYs的信息。最近发布的完全组装的辣椒基因组序列使我们能够对辣椒WRKY蛋白进行全基因组研究。在本研究中，在辣椒基因组中共鉴定出71个WRKY基因。根据其编码蛋白的结构特征，将辣椒WRKY基因（CaWRKY）分为3大类，第2类又分为5个亚类。基因组图谱分析表明，CaWRKY基因在4条染色体上富集，尤其是在1号染色体上，15.5%的家系成员为串联重复基因。根据辣椒和拟南芥的WRKY结构域序列构建系统发育树。通过定量RT-PCR检测了21个CaWRKY基因在盐、热激、干旱、辣椒疫霉（Phytophtora capsici）、水杨酸（SA）、茉莉酸甲酯（MeJA）和脱落酸（阿坝）等7种不同生物和非生物胁迫下的表达情况。本研究为辣椒WRKY基因的功能鉴定和分子育种研究提供了平台。
The WRKY family of transcription factors is one of the most important families of plant transcriptional regulators with members regulating multiple biological processes, especially in regulating defense against biotic and abiotic stresses. However, little information is available about WRKYs in pepper (Capsicum annuum L.). The recent release of completely assembled genome sequences of pepper allowed us to perform a genome-wide investigation for pepper WRKY proteins. In the present study, a total of 71 WRKY genes were identified in the pepper genome. According to structural features of their encoded proteins, the pepper WRKY genes (CaWRKY) were classified into three main groups, with the second group further divided into five subgroups. Genome mapping analysis revealed that CaWRKY were enriched on four chromosomes, especially on chromosome 1, and 15.5% of the family members were tandemly duplicated genes. A phylogenetic tree was constructed depending on WRKY domain' sequences derived from pepper and Arabidopsis. The expression of 21 selected CaWRKY genes in response to seven different biotic and abiotic stresses (salt, heat shock, drought, Phytophtora capsici, SA, MeJA, and ABA) was evaluated by quantitative RT-PCR; Some CaWRKYs were highly expressed and up-regulated by stress treatment. Our results will provide a platform for functional identification and molecular breeding studies of WRKY genes in pepper.