The effect of a peat-based preparation on mitogen-induced proliferation of thymocytes in non-treated and hydrocortisone-suppressed mice.
The effect of a peat-based preparation on mitogen-induced proliferation of thymocytes in non-treated and hydrocortisone-suppressed mice.
复制标题
泥炭制剂对未经治疗和氢化可的松抑制的小鼠中丝裂原诱导的胸腺细胞增殖的影响。
DOI：
10.1078/0944-7113-00028
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发表时间：
2001
期刊：
影响因子：
--
通讯作者：
M. Stefañska
中科院分区：
文献类型：
--
作者：
B. Obminska;M. Stefañska
在用泥炭基制剂（PBP）处理的Balb/c小鼠（5-6周龄）中进行研究，以0.01、0.1或1 mg/kg的剂量以24 h间隔腹膜内给药一次或四次。此外，将氢化可的松以125 mg/kg的单次剂量腹膜内注射至选定的小鼠。结果表明，PBP能暂时增强小鼠胸腺细胞在刀豆球蛋白A（ConA）和植物血凝素（PHA）刺激下的体外增殖能力。PBP的效果取决于后续剂量的数量，但不取决于应用的剂量。单次给予PBP不影响胸腺细胞对ConA和PHA的增殖反应。单次注射PBP（剂量从0.01至1 mg/kg）不会改变胸腺细胞的数量和该器官的重量比。随后PBP注射剂量增加（0.01和0.1 mg/kg）不影响胸腺细胞数量，但在最后一次注射后两天暂时增加胸腺重量比。在氢化可的松之前给予PBP可防止药物对体外用Con A和PHA刺激的胸腺细胞增殖反应的抑制作用，同时与对照组（无氢化可的松）相比，增加胸腺细胞对两种有丝分裂原的增殖反应。单剂量PBP的效果取决于应用的剂量-在0.01 mg/kg的剂量下观察到最弱的预防效果。无论应用的剂量如何，随后PBP剂量的数量增加，都抑制了药物对体外用这些促有丝分裂原刺激的胸腺细胞增殖活性的保护作用。此外，研究中获得的结果表明，PBP部分阻止氢化可的松的抑制作用，因为胸腺细胞的数量和该器官的重量比急剧下降。PBP加速胸腺的再生，但这取决于应用的剂量和后续剂量的数量。当PBP以1 mg/kg的剂量注射4次时，结果最强和最快。PBP引起的胸腺再生很可能与该药对胸腺细胞成熟和分化的影响有关。
The studies were carried out on Balb/c mice (5-6 weeks of age) treated with a peat-based preparation (PBP), administered i.p. once or four times at 24 h intervals at doses of 0.01; 0.1 or 1 mg/kg. Additionally, hydrocortisone was injected i.p. to selected mice at a single dose of 125 mg/kg. The results show that PBP temporarily enhances the proliferative capability of murine thymocytes stimulated in vitro with concanavalin A (Con A) and phytohemagglutinin (PHA). The effect of PBP depends on the number of subsequent doses, but does not depend on the dose applied. A single PBP administration does not affect the proliferative response of thymocytes to Con A and PHA. A single injection of PBP (doses from 0.01 to 1 mg/kg) does not change the number of thymic cells and weight ratio of this organ. Increased doses of subsequent PBP injections (0.01 and 0.1 mg/kg) do not affect the number of thymocytes, but temporarily increase the weight ratio of the thymus two days after the last injection. Administration of PBP prior to hydrocortisone prevents the suppressive effect of the drug on proliferative response of thymocytes stimulated in vitro with Con A and PHA, at the same time increasing the proliferative response of thymic cells to the two mitogens in relation to the control group (hydrocortisone-free). The effect of a single dose of PBP depends on the dose applied--the weakest preventive effect was observed at a dose of 0.01 mg/kg. An increase in the number of subsequent PBP doses, irrespective of a dose applied, prolongs the protective action of the drug on proliferative activity of thymocytes stimulated in vitro with these mitogenes. Moreover, the results obtained in the studies show that PBP partially prevents the suppressive effect of hydrocortisone, as the number of thymic cells and weight ratio of this organ drastically decreased. PBP accelerates regeneration of the thymus, but this depends on a dose applied and the number of subsequent doses. The result was the strongest and the fastest when PBP was injected four times at a dose of 1 mg/kg. It seems quite likely that the thymic regeneration due to PBP is connected with the effect of this drug on maturation and differentiation of thymic cells.