Vertebrate 2xRBD hnRNP proteins: a comparative analysis of genome, mRNA and protein sequences
Vertebrate 2xRBD hnRNP proteins: a comparative analysis of genome, mRNA and protein sequences
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DOI：
10.1016/j.compbiolchem.2004.11.002
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发表时间：
2005-02-01
影响因子：
3.1
通讯作者：
Manzini, G
中科院分区：
文献类型：
--
作者：
Akindahunsi, AA;Bandiera, A;Manzini, G
hnRNP蛋白参与许多细胞功能，主要是前mRNA加工。我们在这里报告的hnRNP家族的2xRBD成员和他们的表达产物的基因的比较分析。从人和鼠来源的七个充分表征的hnRNP成员开始（A0、A1、A2/B1、A3、AB、D和DL）和具有相关RBD串联的三种MuSashI样蛋白（MSI 1、MSI 2和DAZAP 1）的同源基因，经BLAST检索，在斑马鱼基因组中鉴定出12个同源基因，在红鳍东方鲀基因组中鉴定出10个同源基因，可分为3个亚组，每一个都具有高度保守的外显子/内含子结构，与人类和小鼠基因中发现的外显子/内含子结构完美匹配。一个例外是hnRNPA 0基因，这是内含子一致的，在所有四个物种。该分析也支持在cDNA和EST数据库的水平，并在这方面扩展到其他脊椎动物物种，即鸡，非洲爪蟾和热带Silurana。将PHYLIP 3.62包（SEQ BOOT，PROTDIST/DNAIST，NEIGHBOR，CONSENSE）用于所有蛋白质及其CDS和人RBD I和II，以推断这些蛋白质的同源性的相关方面。这些基因中内含子的进化的一些线索来自于它们在其他真核生物同源基因中的分布分析，这些真核生物包括玻璃海鞘、果蝇、小杆线虫、酵母和拟南芥。(c)2004 Elsevier Ltd.保留所有权利。
hnRNP proteins are involved in many cell functions, primarily in pre-mRNA processing. We report here a comparative analysis of the genes of the 2xRBD members of the hnRNP family and of their expression products. Starting from the seven well characterized hnRNP members of human and murine origin (A0, A1, A2/B1, A3, AB, D and DL) and the three MuSashI-like proteins with related RBD tandems (MSI1, MSI2 and DAZAP1), we identified through BLAST search 12 homologous genes in the genome of Danio rerio and 10 in the genome of Takifugu rubripes, which can be divided into three subgroups, each with its highly conserved exon/intron structure, matching perfectly the exon/intron structures found in human and mouse genes. An exception is the gene of hnRNP A0, which is intronless consistently in all the four species. The analysis has been supported also at the level of cDNA and EST databases and extended in this respect to other vertebrate species, namely chicken, Xenopus laevis and Silurana tropicalis. PHYLIP 3.62 package (SEQBOOT, PROTDIST/DNADIST, NEIGHBOR, CONSENSE) was used for all the proteins and their CDSs and human RBDs I and II to infer relevant aspects of the phylogenesis of these proteins. Some clues to the evolution of introns in these genes have come from the analysis of their distribution in homologous genes of other eukaryotes, namely Ciona, Drosophila, Caenorhabditis, Saccharomyces and Arabidopsis. (c) 2004 Elsevier Ltd. All rights reserved.