BON1 interacts with the protein kinases BIR1 and BAK1 in modulation of temperature-dependent plant growth and cell death in Arabidopsis.
BON1 interacts with the protein kinases BIR1 and BAK1 in modulation of temperature-dependent plant growth and cell death in Arabidopsis.
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DOI：
10.1111/j.1365-313x.2011.04659.x
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发表时间：
2011-09
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影响因子：
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通讯作者：
Zaoxia Wang;Pei Meng;Xiaoyan Zhang;D. Ren;Shuhua Yang
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作者：
Zaoxia Wang;Pei Meng;Xiaoyan Zhang;D. Ren;Shuhua Yang
拟南芥copine基因BON 1编码钙依赖性磷脂结合蛋白，参与植物生长稳态和抗病性。然而，BON 1调节植物生长和防御反应的生化和分子机制还没有很好地了解。在这里，我们表明，BON 1相互作用的富含亮氨酸的重复受体样激酶BIR 1（BAK 1相互作用受体样激酶1）和病原体相关的分子模式（PAMP）受体调节BAK 1在体外和体内物理。此外，bon 1和bir 1突变体表现出协同相互作用。虽然bir 1无效突变体与bon 1相比具有相似的生长和细胞死亡缺陷，但bir 1 bon 1双突变体显示出比单突变体更严重的表型。Bon 1 -1和bir 1 -1表型分别被BIR 1和BON 1的过表达部分抑制。此外，bir 1表型通过抗性（R）基因SNC 1（npr 1 -1的抑制子，组成型1）中的功能丧失突变而减弱，SNC 1介导bon 1中的防御反应。有趣的是，BON 1和BIR 1可以在体外被BAK 1磷酸化。我们的研究结果表明，BIR 1作为植物抗性的负调节因子发挥作用，BON 1和BIR 1可能调节PAMP和R蛋白触发的免疫反应。
The Arabidopsis copine gene BON1 encodes a calcium-dependent phospholipid-binding protein involved in plant growth homeostasis and disease resistance. However, the biochemical and molecular mechanisms by which BON1 modulates plant growth and defense responses are not well understood. Here, we show that BON1 interacts physically with the leucine-rich-repeat receptor-like kinases BIR1 (BAK1-interacting receptor-like kinase 1) and pathogen-associated molecular pattern (PAMP) receptor regulator BAK1 in vitro and in vivo. Additionally, bon1 and bir1 mutants exhibit synergistic interaction. While a bir1 null mutant has similar growth and cell-death defects compared with bon1, a bir1 bon1 double mutant displays more severe phenotypes than does the single mutants. The bon1-1 and bir1-1 phenotypes are partially suppressed by overexpression of BIR1 and BON1, respectively. Furthermore, the bir1 phenotype is attenuated by a loss-of-function mutation in the resistance (R) gene SNC1 (Suppressor of npr1-1, constitutive 1), which mediates defense responses in bon1. Intriguingly, BON1 and BIR1 can be phosphorylated by BAK1 in vitro. Our findings suggest that BIR1 functions as a negative regulator of plant resistance and that BON1 and BIR1 might modulate both PAMP- and R protein-triggered immune responses.