Interleukin-1 and IL-1 receptor antagonist in gingival crevicular fluid
Interleukin-1 and IL-1 receptor antagonist in gingival crevicular fluid
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DOI：
10.1034/j.1600-051x.2000.027010738.x
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发表时间：
2000-10-01
影响因子：
6.7
通讯作者：
Fairclough, AL
中科院分区：
文献类型：
--
作者：
Rawlinson, A;Dalati, MHN;Fairclough, AL
背景/目的：本研究旨在探讨成人牙周炎患者龈沟液（GCF）中细胞因子IL-IP及其受体拮抗剂IL-1ra的变化。方法：收集10例中重度成人牙周炎患者和10例健康对照者共40份GCF样本。受试者的性别不限，上门牙全部存在，无相关全身性疾病、妊娠或近期用药。所有受试者均为非吸烟者，且在前3个月内未接受任何牙周治疗。研究上前牙深部出血部位、深部非出血部位和健康部位的关系。在获得GCF样本后，记录每个部位的临床测量。使用新的市售ELISA试剂盒（Quantikine(TM)）定量IL-1 β和IL-1ra，并可在所有样品中检测到。结果：出血牙周炎部位IL-1 β的平均浓度为0.11 (SD 0.14) pg/mu l，非出血牙周炎部位IL-1 β的平均浓度为0.04 (0.05)pg/mu l，健康部位IL-1 β的平均浓度为0.01 (0.03)pg/mu l
Background/aims: This study aimed to investigate the cytokine IL-IP and its receptor antagonist IL-1ra in gingival crevicular fluid (GCF), in patients with adult periodontitis.Method: A total of 40 GCF samples were harvested from 10 subjects with moderate to severe adult periodontitis and 10 healthy controls. Subjects were selected fr om both genders, with all the upper anterior teeth present, and with no relevant systemic illness, pregnancy or recent medication. All subjects were non-smokers and had not received any periodontal therapy within the preceding 3 months. Deep bleeding sites, deep non-bleeding sites and healthy sites were investigated in relation to upper anterior teeth. Clinical measurements were recorded for each site, after obtaining a GCF sample. IL-1 beta and IL-1ra were quantified using new commercially available ELISA kits (Quantikine(TM)), and could be detected in all samples.Results: The mean concentration for IL-1 beta was 0.11 (SD 0.14) pg/mu l for bleeding periodontitis sites, 0.04 (0.05) pg/mu l for non-bleeding periodontitis sites, and 0.01 (0.03) pg/mu l for healthy sites (p