Cooperative nature of the binding of cholesterol on to synaptosomal plasma membranes of dog brain.
Cooperative nature of the binding of cholesterol on to synaptosomal plasma membranes of dog brain.
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胆固醇与狗脑突触体质膜结合的协同性质。
DOI：
10.1016/0005-2736(81)90360-6
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发表时间：
1981
期刊：
影响因子：
--
通讯作者：
G. Theodosiadis
中科院分区：
文献类型：
--
作者：
S. Alivisatos;G. Deliconstantinos;A. Papaphilis;G. Theodosiadis
研究了胆固醇水溶液中胆固醇与狗脑突触体质膜的“结合”。外源胆固醇的“结合”是一个缓慢的过程，严格取决于胆固醇的浓度和存在的膜的数量。看来结合可能发生在三个不同的连续阶段。第一阶段发生得非常快，包括大量胆固醇沉积在细胞膜上。这一阶段的特点是缺乏整合蛋白的功能变化。随后或伴随着一种较慢的结合，可能是在“特定的结合位点”，其本质很可能是合作的。因此，当胆固醇葡糖苷以与胆固醇相比较低的浓度使用时，它会增加胆固醇的结合，而在相对于胆固醇较高的浓度下，它会拮抗其结合。这一阶段会引起整合蛋白的强烈功能变化，不间断地合并，将胆固醇作为结构元素纳入膜框架（非特异性结合）。
The ‘binding’ of cholesterol on to dog brain synaptosomal plasma membranes from aqueous cholesterol ‘solutions’ was studied. ‘Binding’ of exogenous cholesterol is a slow process, strictly depending on the concentration of cholesterol and the quantity of the membranes present. It appears that binding probably occurs in three distinct successive stages. The first stage occurs very rapidly, and consists of a large deposition-like accumulation of cholesterol onto the membranes. This stage is characterized by the lack of functional changes of integral proteins. It is followed or accompanied by a slower type of binding, probably at ‘specific binding sites’, the nature of which is, in all probability, cooperative. Thus, when the glucoside of cholesterol is used at lower concentrations as compared to cholesterol it increases the binding of cholesterol, while at higher concentrations relative to cholesterol, it antagonizes its binding. This stage, which evokes strong functional changes of integral proteins, merges without interruption into an incorporation of cholesterol as a structural element into the membranous framework (nonspecific binding).