DNA damage enhancement from gold nanoparticles for clinical MV photon beams.
DNA damage enhancement from gold nanoparticles for clinical MV photon beams.
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DOI：
10.1667/rr3001.1
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发表时间：
2012-12
影响因子：
3.4
通讯作者：
Makrigiorgos GM
中科院分区：
文献类型：
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作者：
Berbeco RI;Korideck H;Ngwa W;Kumar R;Patel J;Sridhar S;Johnson S;Price BD;Kimmelman A;Makrigiorgos GM
在这项研究中，我们量化了由于金纳米颗粒(GNP)存在的相对损伤增强在临床6 MV射束在不同的输送参数和深度。预计产生较大比例低能光子的深度和输送方式将对含有GNP的细胞样本产生更大的影响。照射深度分别为1.5、5、10、15和20 cm。采用等心方孔径为5、10、15 cm的常规光束和平坦的无滤光片(FFF)光束。γ-H_2AX染色评价硝普钠对DNA损伤的相对增强作用。与没有GNP相比，有GNP的DNA损伤在所有深度和投放方式下都有统计学意义的增加。相对于最浅的深度，观察到所有交付的损伤增强都随着深度的增加而增加。对于传统的(露天)投放，GNP对DNA损伤的增强作用被认为是随场大小的增加而增加的。对于FFF交付，与传统的现场交付相比，增强效果显著增加。测量的相对DNA损伤增强验证了作为临床MV光子束的深度和传递方式的函数的理论预测的趋势。这项研究结果为金纳米颗粒辅助放射治疗的临床开发开辟了新的可能性。
In this study, we quantify the relative damage enhancement due to the presence of gold nanoparticles (GNP) in vitro in a clinical 6 MV beam for various delivery parameters and depths. It is expected that depths and delivery modes that produce a larger proportions of low-energy photons will have a larger effect on the cell samples containing GNP. HeLa cells with and without 50 nm GNP were irradiated at depths of 1.5, 5, 10, 15 and 20 cm. Conventional beams with square aperture sizes 5, 10 and 15 cm at isocenter, and flattening filter free (FFF) beams were used. Relative DNA damage enhancement with GNP was evaluated by γ-H2AX staining. Statistically significant increases in DNA damage with GNP, compared to the absence of GNP, were observed for all depths and delivery modes. Relative to the shallowest depth, damage enhancement was observed to increase as a function of increasing depth for all deliveries. For the conventional (open field) delivery, DNA damage enhancement with GNP was seen to increase as a function of field size. For FFF delivery, a substantial increase in enhancement was found relative to the conventional field delivery. The measured relative DNA damage enhancement validates the theoretically predicted trends as a function of depth and delivery mode for clinical MV photon beams. The results of this study open new possibilities for the clinical development of gold nanoparticle-aided radiation therapy.