Detection of viral bioagents using a shear horizontal surface acoustic wave biosensor
Detection of viral bioagents using a shear horizontal surface acoustic wave biosensor
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DOI：
10.1016/j.bios.2007.12.016
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发表时间：
2008-04-01
影响因子：
12.6
通讯作者：
Larson, R. S.
中科院分区：
文献类型：
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作者：
Bisoffi, M.;Hjelle, B.;Larson, R. S.
病毒是高度医学和生物防御关注的问题，并且在远低于引起健康危害所需的阈值的浓度下检测它们在灵敏度、特异性和选择性方面仍然是一个挑战。理想地，用于病毒因子的准确和真实的时间检测的测定将不需要分析物的任何预处理，这将使它们适用于例如体液（血液、痰）和人造以及天然存在的水体（水池、河流）。我们在本文中描述了一种稳健的生物传感器，其将在325 MHz频率下产生的表面声波（SAW）的灵敏度与抗体提供的用于检测病毒因子的特异性相结合。钽酸锂基SAW换能器与二氧化硅波导传感器平台，具有三个测试和一个参考延迟线，用于吸附抗体针对科萨基病毒134或A类生物因子辛诺布尔病毒（SNV），汉坦病毒属成员，布尼亚病毒科，负链RNA病毒。对于两种病毒，快速检测（在几秒钟内）增加的病毒颗粒浓度在数量级范围内呈线性，尽管传感器对于SNV的检测灵敏度约为5 × 10（5）倍。对于这两种病原体，传感器对其目标的选择性不会受到混杂单纯疱疹病毒的影响。1型病毒。该生物传感器能够在低于通常在患有汉坦病毒心肺综合征（HCPS）的人类患者中发现的病毒载量的剂量下检测SNV。此外，在原理证明的真实的世界应用中，SAW生物传感器能够在没有分析物预处理的情况下选择性地检测复杂溶液（例如天然存在的水体（河流、污水流出物））中的SNV试剂。这是第一项研究，报告使用抗体为基础的SAW生物传感器，有可能被用作一个手持和独立的设备，在现场进行快速病毒检测的病毒剂的检测。(C)2007 Elsevier B.V.保留所有权利。
Viruses are of high medical and biodefense concern and their detection at concentrations well below the threshold necessary to cause health hazards continues to be a challenge with respect to sensitivity, specificity, and selectivity. Ideally, assays for accurate and real time detection of viral agents would not necessitate any pre-processing of the analyte, which would make them applicable for example to bodily fluids (blood, sputum) and man-made as well as naturally occurring bodies of water (pools, rivers). We describe herein a robust biosensor that combines the sensitivity of surface acoustic waves (SAW) generated at a frequency of 325 MHz with the specificity provided by antibodies for the detection of viral agents. A lithium tantalate-based SAW transducer with silicon dioxide waveguide sensor platform featuring three test and one reference delay lines was used to adsorb antibodies directed against either Coxsackie virus 134 or the category A bioagent Sin Nombre virus (SNV), a member of the genus Hantavirus, family Bunyaviridae, negative-stranded RNA viruses. Rapid detection (within seconds) of increasing concentrations of viral particles was linear over a range of order of magnitude for both viruses, although the sensor was approximately 5 x 10(5)-fold more sensitive for the detection of SNV. For both pathogens, the sensor's selectivity for its target was not compromised by the presence of confounding Herpes Simplex. virus type 1. The biosensor was able to detect SNV at doses lower than the load of virus typically found in a human patient suffering from hantavirus cardiopulmonary syndrome (HCPS). Further, in a proof-of-principle real world application, the SAW biosensor was capable to selectively detect SNV agents in complex solutions, such as naturally occurring bodies of water (river, sewage effluent) without analyte pre-processing. This is the first study that reports on the detection of viral agents using an antibody-based SAW biosensor that has the potential to be used as a hand-held and self-contained device for rapid viral detection in the field. (C) 2007 Elsevier B.V. All rights reserved.